Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Anti-wrinkle effects of solvent fractions from Jubak on CCD-986sk

CCD-986sk 세포 내 주박 분획물의 항주름 효능

  • Young-Ah Jang (Division of Cosmetic Science and Technology, Daegu Haany University) ;
  • Hyejeong Lee (College of Pharmacy, Duksung Women's University)
  • 장영아 (대구한의대학교 화장품공학부) ;
  • 이혜정 (덕성여자대학교 약학대학)
  • Received : 2024.03.19
  • Accepted : 2024.04.29
  • Published : 2024.04.30
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Abstract

In this study, in order to evaluate the possibility of using Jubak as a functional cosmetic material, evaluation of antioxidant activity according to fractions and anti-wrinkle efficacy in CCD-986sk cells, a human fibroblast, were conducted. As a result of confirming the antioxidant activity by measuring ABTS+ radical scavenging ability, Jubak's Ethyl Acetate fractions was found to be 75.5% at a concentration of 1,000 ㎍/ml, showing the highest antioxidant activity among the extraction solvents. The wrinkle improvement effect was confirmed by measuring the inhibitory activity of elastase and collagenase, and in both test results, Jubak's Ethyl Acetate fractions showed the highest efficacy at a concentration of 1,000 ㎍/ml. As a result of measuring the synthesis rate of pro-collagen type I in CCD-986sk cells induced by UVB, Jubak showed the highest efficacy in the order of Ethyl Acetate, Water, Acetonitrile, and Hexan fractions at the same concentration of 20 ㎍/ml. As a result of measuring the inhibition rate of MMP-1, a collagen degrading enzyme, all four solvent fractions showed an efficacy of more than 70% at 20 ㎍/ml. As a result of measuring the mRNA expression levels of pro-collagen type I, MMP-1, and MMP-3 in a real-time PCR experiment, the protein expression level of pro-collagen type I increased when treated with Jubak fractions compared to the UVB group alone. The mRNA expression levels of MMP-1 and MMP-3 were confirmed to be decreased, and Ethyl Acetate fractions was the most effective in improving wrinkles after the control group (EGCG). As a result, it was confirmed that the Ethyl Acetate fractions among Jubak's solvent fractions has an anti-wrinkle effect against photoaging caused by UVB stimulation, and is expected to be used as a natural material for cosmetics.

본 연구에서는 주박의 기능성 화장품 소재로서의 활용 가능성을 평가하기 위해 분획물에 따른 항산화 활성평가와 인간섬유아세포인 CCD-986sk 세포에서의 주름효능 평가를 진행하였다. ABTS+ radical 소거능 측정을 통해 항산화 활성을 확인한 결과, 주박 Ethyl Acetate 분획물 1,000 ㎍/ml 농도에서 75.5%로 나타나 분획 용매 중 가장 높은 항산화 활성을 보였다. Elastase, Collagenase 저해 활성 측정을 통해 주름 개선 효과를 확인하였으며, 두 실험 결과 모두 주박 Ethyl Acetate 분획물이 1,000 ㎍/ml 농도에서 가장 높은 효능을 나타내었다. UVB로 유도된 CCD-986sk 세포 내 pro-collagen type I의 합성율을 측정한 결과 주박은 동일농도에서 20 ㎍/ml에서 Ethyl Acetate, Water, Acetonitrile, Hexan 분획물 순으로 높은 효능을 보였다. 콜라겐 분해효소인 MMP-1의 저해률을 측정한 결과 4가지 용매 분획몰은 20 ㎍/ml에서 모두 70% 이상의 효능을 나타내었다. Real time PCR 실험에서 pro-collagen type I, MMP-1 및 MMP-3의 mRNA 발현량을 측정한 결과 UVB 단독군 대비 Jubak 추출물을 함께 처리했을 때 pro-collagen type I의 단백질 발현량이 증가되었고, MMP-1과 MMP-3의 mRNA 발현량은 감소하는 것을 확인하였으며 대조군(EGCG) 다음으로 Ethyl Acetate 추출물이 주름 개선에 가장 효능이 우수하였다. 이상의 결과로 주박의 용매 분획물 중 Ethyl Acetate 분획물은 UVB 자극에 의한 광노화에 대한 항주름 효과가 있음을 확인하여 화장품의 천연물 소재로서의 이용이 기대된다.

Keywords

Acknowledgement

본 연구는 2023년도 덕성여자대학교 교내연구비 지원으로 이루어졌음(2023-3000008127)

References

  1. J. H. Jeong, "한국인의 피부노화 특징 및 발생기전", Journal of the society of cosmetic scientists of Korea, Vol.27, No.2, pp. 31-35, (2001). 
  2. S. H. Hong, H. N. Lee, T. K. Jeong, Y. H. Kim, H. S. Jeong, J. S. Lee, "Protective Effect of Jujube Water Extract against UVB-Induced Skin Photoaging in Human Skin Fibroblasts", Journal of the Korean Society of Food Science and Nutrition, vol. 48, No. 10, pp. 1172-1177, (2019). 
  3. K. H. Lee, H. S. Park, l. J. Yoon, Y. B. Shin, Y. C. Baik, D. H. Koo, S. K. Kim, H. K. Jung, M. O. Sim, H. W. Cho, W. S. Jung, M. S. Kim "Whitening and Anti-Wrinkle Effects of Tremella Fuciformis Extracts", Korean Journal of Medicinal Crop Science, Vol. 24, pp. 38-46, (2016). 
  4. Nagase, Hideaki, "Matrix metalloproteinases." Zinc Metalloproteases in Health and Disease, pp.173-224, (1996). 
  5. S. C. Lim, "Expression of matrix metalloproteinases and its inhibitor in gastric adenocarcinoma", Cancer Research and Treatment, Vol. 33, No. 3 pp. 199-206, (2001). 
  6. J. S. Roh, H. J. Yeom, M. J. Oh, J. Y. Lee, "A Study on the Antioxidant and MMPs Protein Expression Inhibitive Effect of Punica granatum L. Extract and Its Stabilization with Liquid Crystal Emulsion", Journal of Life Science, Vol. 31, No. 2 pp. 164-174, (2021). 
  7. S. Kim, T. j. Park, J. S. Lim, S. Y. Kim, "Effect of Agaricus biorenovate Extract on Collagen Synthesis and Matrix Metalloproteinase-1 Production in Human Dermal Fibroblast" KSBB Journal, Vol. 34, No. 1, pp. 49-53, (2019). 
  8. E. H. Lee, S. Y. Lee, H. Y. Jung, I. K. Kang, D. H. Ahn, Y. J. Cho, "Photo-aging regulation effects of newly bred Green ball apple", Journal of Applied Biological Chemistry, Vol. 63, No. 1, pp. 75-82, (2020). 
  9. S. M. Lee, S. J. Lee, Y. Y. Kwon, S. H. Baek, J. S. Kim, H. Y. Sohn, W. C. Shin, "Skin whitening and anti-wrinkle effects of extract from Jubak of oriental herbal liquor", Journal of the Korean Society of Food Science and Nutrition, Vol. 43, No. 11, pp. 1695-1700, (2014). 
  10. M. S. Kim, D. K. Kang, W. C. Shin, H. Y. Sohn, "Anti-microbial, anti-oxidant, and anti-thrombosis activities of the lees of Bokbunja wine (Rubus coreanus Miquel)", Journal of Life Science, Vol. 25, No. 7, pp. 757-764, (2015). 
  11. S. M. Kim, E. H. Kim, D. H. Song, Y. S. Jung, H. J. Jeon, Y. K. Choi, H. J. Kim, "Extraction and Fermentation of Rice Wine Lees for the Development of Cosmetic Ingredients", Journal of the Korean Society of Cosmetology, Vol. 19, No. 3, pp. 501-508, (2013). 
  12. R. L. Prior, X. Wu, K. Schaich, "Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements", Journal of agricultural and food chemistry, Vol.53, No.10, pp. 4290-4302, (2005). 
  13. M. J. Jang, D. H. Jun, S. H. Kim, S. I. Han, J. T. Lee, "Anti-wrinkle Effect of Chestnut Leaf", Journal of Life Science, Vol.23, No.6, pp. 751-756, (2013). 
  14. D. H. Kim, T. S. Park, J. H. Son, "Anti-wrinkle Activities Verification of Buplerum falcatum Extracts on CCD-986sk", Journal of Applied Biological Chemistry, Vol.58, No.2, pp. 183-187, (2015). 
  15. W. Y. Jung, J. M. Jeong, "Change of antioxidative activity at different harvest time and improvement of atopic dermatitis effects for persimmon leaf extract", The Korea Journal of Herbology, Vol. 27, No. 1, pp. 41-49, (2012). 
  16. Y. B. Cho, J. Y. Kim, N. W. Kwon, B. Y. Hwang, J. G. Kim, S. H. Woo, M. S. Lee, "Purification and Identification of Cytotoxic Compounds from the Root of Rumex crispus L.", Korean Journal of Medicinal Crop Science, Vol. 27, No. 3, pp. 208-217, (2019). 
  17. Park hye ryeon, & AHN HONG SEOK, "Effect of Procollagen Type I Stimulation and Inhibiton of MMP-2 mRNA Expression from Chenopodium album Ethanol Extract", Journal of the Korean Society of Cosmetology, Vol. 21, No. 6, pp. 1189-1195, (2015). 
  18. D. H, Kim, T. S. Park, J. H. Son, "Potential prevention effects of Rubus occidentalis seed on UVB-induced MMP-1 production and procollagen degradation in CCD-986sk cells", Journal of Applied Biological Chemistry, Vol. 59, No. 4, pp. 317-322, (2016). 
  19. J. E. Kim, Y. S. Choi, H. K. Kim, Y. A. Jang, "Collagen synthesis ability and inhibitory effect of MMPs in keratinocytes of Lysimachia christinae Hance Extract", The Korean Society of Applied Science and Technology, Vol. 37, No. 4, pp. 820-829, (2020). 
  20. Y. A. Jang, "A Verification of Cosmetic Effect about Anti-oxidant and Anti-wrinkle of 11 Native Plants", Journal of Life Science, Vol. 26, No. 7, pp. 782-788, (2016). 
  21. S. H. Lee, D. Y. Choi, Y. H. Lee, "Response to Bee Venom Acupuncture and Polymorphism of Matrix Metalloproteinase-1 Gene in Korean Patients with Rheumatoid Arthritis", Journal of Acupuncture Research, Vol .21, No. 1, pp. 211-225, (2004). 
  22. Y. A. Jang, J. T. Lee, "Anti-wrinkle effect of berberine by inhibition of MMP-2 and MMP-9 activity in fibroblasts", Journal of Applied Biological Chemistry, Vol. 61, No. 1, pp. 9-15, (2018). 
  23. B. A. Kim, "Anti-aging effect of Codium fragile extract on keratinocytes damaged by fine dust PM10", The Korea Jounrnal of Herbology, Vol. 38, No. 4, pp. 45-52, (2023).