Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Skin permeability of the biosynthetic galactosylated 2-phenoxyethanol

생합성된 galactosylated 2-phenoxyethanol의 피부 투과도

  • Su-Hong Kim (Department of Biotechnology, Korea National University of Transportation) ;
  • Sun-Beom Kwon (Department of Biotechnology, Korea National University of Transportation) ;
  • Jun-Sub Kim (Department of Biotechnology, Korea National University of Transportation) ;
  • Gi-Seong Moon (Department of Biotechnology, Korea National University of Transportation) ;
  • Kyung-hwan Jung (Department of Biotechnology, Korea National University of Transportation) ;
  • Hyang-Yeol Lee (Department of Biotechnology, Korea National University of Transportation)
  • 김수홍 (한국교통대학교 식품생명학부 생명공학전공) ;
  • 권순범 (한국교통대학교 식품생명학부 생명공학전공) ;
  • 김준섭 (한국교통대학교 식품생명학부 생명공학전공) ;
  • 문기성 (한국교통대학교 식품생명학부 생명공학전공) ;
  • 정경환 (한국교통대학교 식품생명학부 생명공학전공) ;
  • 이향렬 (한국교통대학교 식품생명학부 생명공학전공)
  • Received : 2023.12.26
  • Accepted : 2024.02.05
  • Published : 2024.02.28
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Abstract

The safety of cosmetic ingredients is considered paramount. In order to enhance safety, a novel preservative, PE-gal, was bio-synthesized by utilizing the Escherichia coli enzyme 𝛽-galactosidase on the conventional preservative 2-phenoxyethanold (PE). The skin absorption of the bio-synthesized product, PE-gal, intended for use in cosmetics, was evaluated for permeability using the Franz Diffusion Cell Assay system, comparing it with the conventinal preservative PE. When using samples of the same mass concentration, the Flux and Kp values of PE increased over time, indicating a gradual increase in permeability. However, PE-gal did not exhibit sufficient permeability to measure. This suggests that the skin permeability of PE is higher than that of the PE-gal saccharide. According to Marzulli et al., when confirming the degree of permeation using Kp values, the permeation rate of PE was measured as "slow" at a concentration of 1mg/mL. Thus, the transdermal permeability of the divedened form of PE-gal was significantly lower compared to PE.

화장품 소재는 안전성이 무엇보다 중요시된다. 화장품에 사용되는 기존의 보존제인 PE에 대장균 효소 𝛽-gal을 이용하여 안전성이 증가된 PE-gal을 생합성하였다. 화장품 소재로 사용하기 위해 생합성된 생성물인 PE-gal의 피부 흡수도를 기존의 보존제인 PE와 비교하기 위해 Franz Diffusion cell Assay 시스템을 이용하여 경피투과도를 측정하였다. 같은 질량농도의 시료를 사용하였을 때 PE의 Flux 값, Kp 값은 시간이 지날수록 증가하는 것으로 나타났으나 PE-gal은 투과도를 측정할 수 있을 만큼 투과되지 못하였다. 이는 PE의 피부투과도가 생합성된 PE-gal 배당체보다 높다는 것을 나타낸다. Marzulli 등에 따라 Kp 값을 이용하여 투과 정도를 확인하였을 때 PE의 투과속도는 1mg/mL의 농도에서 느림(slow)으로 측정되었다. 따라서 배당체 형태의 PE-gal은 PE에 비해 경피 투과도가 현저히 낮게 나타났다.

Keywords

Acknowledgement

본 과제(결과물)는 2023년도 교육부의 재원으로 한국연구재단의 지원을 받아 수행된 지자체-대학 협력기반 지역혁신 사업의 결과입니다.(2021RIS-001)

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