Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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Description of unrecorded wild yeasts from soil in Republic of Korea under cold conditions

  • Soohyun Maeng (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Sathiyaraj Srinivasan (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
  • Received : 2023.11.15
  • Accepted : 2023.12.14
  • Published : 2024.05.31
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Abstract

The purpose of this study was to isolate and identify wild yeasts from soil collected in Daegu City and Cheongyang County, Republic of Korea. Among 11 strains isolated in this study, nine strains were previously reported and two strains were unreported in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation test are done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. Of the two unrecorded yeast strains, CY-9-10C belongs to the genus Mrakia (family Mrakiaceae, order Cystofilobasidiales, class Tremellomycetes) and PG3-4-10C belongs to the genus Slooffia (family Chrysozymaceae, order Microbotryomycetes incertae sedis, class Microbotryomycetes). Both strains had oval-shaped and polar budding cells. This research described the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (No.2021R1F1A1061389), also supported by National Institute of Biological Resources (NIBR) (2022M3H9A1082984) and a grant from the National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202304104).

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