대한소아치과학회지 (Journal of the korean academy of Pediatric Dentistry)

  • 제51권2호
  • /
  • Pages.165-175
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  • 2024
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  • 1226-8496(pISSN)
  • /
  • 2288-3819(eISSN)
대한소아치과학회 (Korean Academy of Pediatric Dentistry)
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Effect of Blood Contamination on Vickers Microhardness and Surface Morphology of Mineral Trioxide Aggregate

  • Jaehyun Seung (Department of Pediatric Dentistry, College of Dentistry, Wonkwang University) ;
  • Seong-Jin Shin (Institute of Tissue Engineering, Dankook University) ;
  • Byounghwa Kim (Department of Pediatric Dentistry, College of Dentistry, Wonkwang University) ;
  • Ji-Myung Bae (Department of Dental Biomaterials and the Institute of Biomaterial and Implant, College of Dentistry, Wonkwang University) ;
  • Jiyoung Ra (Department of Pediatric Dentistry, College of Dentistry, Wonkwang University)
  • 투고 : 2024.03.01
  • 심사 : 2024.04.10
  • 발행 : 2024.05.31
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초록

This study aimed to investigate the effects of blood contamination on the Vickers hardness and the surface morphology of premixed MTA and compare them with the effects on conventional MTA. The Vickers microhardness of Endocem MTA Premixed Regular (EP) and ProRoot MTA (PM) was assessed after immersion in fetal bovine serum (FBS) and saline. Stem cells from human exfoliated deciduous teeth (SHED) were seeded on MTA after immersion in FBS, saline, and deionized water (DW). Cell adhesion patterns and surface morphology were visualized via scanning electron microscopy (SEM). The surface microhardness of EP and PM in FBS was lower than in saline. However, short-term exposure of PM to FBS did not reduce the microhardness compared to saline. Angular crystals formed in water, while rounded crystals with more air voids appeared in FBS. Favorable SHED attachment occurred in all groups. Overall, the surface hardness of EP and PM decreased after FBS exposure, although PM was less influenced. We suggest minimizing the amount of bleeding when using MTA clinically; nevertheless, PM remains an option with more expected blood contamination than EP. In summary, exposure to FBS decreased mechanical performance but allowed cell adhesion for both MTAs, with PM being more resistant to these changes.

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참고문헌

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