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Effect of Blood Contamination on Vickers Microhardness and Surface Morphology of Mineral Trioxide Aggregate

  • Jaehyun Seung (Department of Pediatric Dentistry, College of Dentistry, Wonkwang University) ;
  • Seong-Jin Shin (Institute of Tissue Engineering, Dankook University) ;
  • Byounghwa Kim (Department of Pediatric Dentistry, College of Dentistry, Wonkwang University) ;
  • Ji-Myung Bae (Department of Dental Biomaterials and the Institute of Biomaterial and Implant, College of Dentistry, Wonkwang University) ;
  • Jiyoung Ra (Department of Pediatric Dentistry, College of Dentistry, Wonkwang University)
  • Received : 2024.03.01
  • Accepted : 2024.04.10
  • Published : 2024.05.31

Abstract

This study aimed to investigate the effects of blood contamination on the Vickers hardness and the surface morphology of premixed MTA and compare them with the effects on conventional MTA. The Vickers microhardness of Endocem MTA Premixed Regular (EP) and ProRoot MTA (PM) was assessed after immersion in fetal bovine serum (FBS) and saline. Stem cells from human exfoliated deciduous teeth (SHED) were seeded on MTA after immersion in FBS, saline, and deionized water (DW). Cell adhesion patterns and surface morphology were visualized via scanning electron microscopy (SEM). The surface microhardness of EP and PM in FBS was lower than in saline. However, short-term exposure of PM to FBS did not reduce the microhardness compared to saline. Angular crystals formed in water, while rounded crystals with more air voids appeared in FBS. Favorable SHED attachment occurred in all groups. Overall, the surface hardness of EP and PM decreased after FBS exposure, although PM was less influenced. We suggest minimizing the amount of bleeding when using MTA clinically; nevertheless, PM remains an option with more expected blood contamination than EP. In summary, exposure to FBS decreased mechanical performance but allowed cell adhesion for both MTAs, with PM being more resistant to these changes.

Keywords

References

  1. Torabinejad M, Hong CU, McDonald F, Pitt Ford TR : Physical and chemical properties of a new root-end filling material. J Endod, 21:349-353, 1995. https://doi.org/10.1016/S0099-2399(06)80967-2
  2. Margunato S, Tasli PN, Aydin S, Karapinar Kazandag M, Sahin F : In Vitro Evaluation of ProRoot MTA, Biodentine, and MM-MTA on Human Alveolar Bone Marrow Stem Cells in Terms of Biocompatibility and Mineralization. J Endod, 41:1646-1652, 2015. https://doi.org/10.1016/j.joen.2015.05.012
  3. Zhu C, Ju B, Ni R : Clinical outcome of direct pulp capping with MTA or calcium hydroxide: a systematic review and meta-analysis. Int J Clin Exp Med, 8:17055-17060, 2015.
  4. Baroudi K, Samir S : Sealing ability of MTA used in perforation repair of permanent teeth; literature review. Open Dent J, 10:278-286, 2016. https://doi.org/10.2174/1874210601610010278
  5. Asgary S, Eghbal MJ, Shahravan A, Saberi E, Baghban AA, Parhizkar A : Outcomes of root canal therapy or full pulpotomy using two endodontic biomaterials in mature permanent teeth: a randomized controlled trial. Clin Oral Investig, 26:3287-3297, 2022. https://doi.org/10.1007/s00784-021-04310-y
  6. Taha NA, Khazali MA : Partial Pulpotomy in Mature Permanent Teeth with Clinical Signs Indicative of Irreversible Pulpitis: A Randomized Clinical Trial. J Endod, 43:1417-1421, 2017. https://doi.org/10.1016/j.joen.2017.03.033
  7. Smail-Faugeron V, Glenny AM, Courson F, Durieux P, Muller-Bolla M, Fron Chabouis H : Pulp treatment for extensive decay in primary teeth. Cochrane Database Syst Rev, 5:CD003220, 2018.
  8. Abd Al Gawad RY, Hanafy RMH : Success rate of three capping materials used in pulpotomy of primary molars: A randomized clinical trial. Saudi Dent J, 33:560-567, 2021. https://doi.org/10.1016/j.sdentj.2020.08.007
  9. Parirokh M, Torabinejad M : Mineral trioxide aggregate: a comprehensive literature review - Part I: chemical, physical, and antibacterial properties. J Endod, 36:16-27, 2010. https://doi.org/10.1016/j.joen.2009.09.006
  10. Abu-Nawareg M, Zidan A : Modification of Portland cement properties using glycerin. Int J Health Sci Res, 10:168-178, 2020.
  11. Back S, Jang YJ, Lee J, Lee J, Shin J, Kim J, Han M, Kim JS : pH, Ion Release Capability, and Solubility Value of Premixed Mineral Trioxide Aggregates. J Korean Acad Pediatr Dent, 49:379-391, 2022. https://doi.org/10.5933/JKAPD.2022.49.4.379
  12. Han L, Kodama S, Okiji T : Evaluation of calcium-releasing and apatite-forming abilities of fast-setting calcium silicate-based endodontic materials. Int Endod J, 48:124-130, 2015. https://doi.org/10.1111/iej.12290
  13. Ber BS, Hatton JF, Stewart GP : Chemical modification of proroot mta to improve handling characteristics and decrease setting time. J Endod, 33:1231-1234, 2007. https://doi.org/10.1016/j.joen.2007.06.012
  14. Nazari A, Riahi S : The effects of ZrO2 nanoparticles on physical and mechanical properties of high strength self compacting concrete. Mater Res, 13:551-556, 2010. https://doi.org/10.1590/S1516-14392010000400019
  15. Cho SY, Park S, Shin Y, Jung IY : Randomized clinical trial of pulpotomy using a premixed injectable calcium silicate cement on mature permanent teeth with reversible pulpitis. Sci Rep, 14:2994, 2024.
  16. Joo Y, Lee T, Jeong SJ, Lee JH, Song JS, Kang CM : A randomized controlled clinical trial of premixed calcium silicate-based cements for pulpotomy in primary molars. J Dent, 137:104684, 2023.
  17. Alqahtani AS, Alsuhaibani NN, Sulimany AM, Bawazir OA : NeoPutty® versus NeoMTA 2® as a pulpotomy medicament for primary molars: A randomized clinical trial. Pediatr Dent, 45:240-244, 2023.
  18. Gartner AH, Dorn SO : Advances in endodontic surgery. Dent Clin North Am, 36:357-378, 1992. https://doi.org/10.1016/S0011-8532(22)02501-0
  19. Nekoofar MH, Oloomi K, Sheykhrezae MS, Tabor R, Stone DF, Dummer PM : An evaluation of the effect of blood and human serum on the surface microhardness and surface microstructure of mineral trioxide aggregate. Int Endod J, 43:849-858, 2010. https://doi.org/10.1111/j.1365-2591.2010.01750.x
  20. Nekoofar MH, Stone DF, Dummer PM : The effect of blood contamination on the compressive strength and surface microstructure of mineral trioxide aggregate. Int Endod J, 43:782-791, 2010. https://doi.org/10.1111/j.1365-2591.2010.01745.x
  21. Tingey MC, Bush P, Levine MS : Analysis of mineral trioxide aggregate surface when set in the presence of fetal bovine serum. J Endod, 34:45-49, 2008. https://doi.org/10.1016/j.joen.2007.09.013
  22. Torabinejad M, Higa RK, McKendry DJ, Pitt Ford TR : Dye leakage of four root end filling materials: effects of blood contamination. J Endod, 20:159-163, 1994. https://doi.org/10.1016/S0099-2399(06)80326-2
  23. Vanderweele RA, Schwartz SA, Beeson TJ : Effect of blood contamination on retention characteristics of MTA when mixed with different liquids. J Endod, 32: 421-424, 2006. https://doi.org/10.1016/j.joen.2005.09.007
  24. Salem Milani A, Rahimi S, Froughreyhani M, Vahid Pakdel M : Effect of Blood Contamination on Marginal Adaptation and Surface Microstructure of Mineral Trioxide Aggregate: A SEM Study. J Dent Res Dent Clin Dent Prospects, 7:157-163, 2013.
  25. Park M, Kim J, Choi N, Kim S : Effect of Blood Contamination on the Push-Out Bond Strength and Surface Morphology of Tricalcium Silicate Materials. J Korean Acad Pediatr Dent, 43:36-43, 2016. https://doi.org/10.5933/JKAPD.2016.43.1.36
  26. Kim Y, Kim S, Shin YS, Jung IY, Lee SJ : Failure of setting of mineral trioxide aggregate in the presence of fetal bovine serum and its prevention. J Endod, 38: 536-540, 2012. https://doi.org/10.1016/j.joen.2011.12.005
  27. Gandolfi MG, Iacono F, Agee K, Siboni F, Tay F, Pashley DH, Prati C : Setting time and expansion in different soaking media of experimental accelerated calcium-silicate cements and ProRoot MTA. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 108:E39-45, 2009. https://doi.org/10.1016/j.tripleo.2009.07.039
  28. Walsh RM, Woodmansey KF, Glickman GN, He J : Evaluation of compressive strength of hydraulic silicate-based root-end filling materials. J Endod, 40:969- 972, 2014. https://doi.org/10.1016/j.joen.2013.11.018
  29. Ramani A, Sangwan P, Tewari S, Duhan J, Mittal S, Kumar V : Comparative evaluation of complete and partial pulpotomy in mature permanent teeth with symptomatic irreversible pulpitis: A randomized clinical trial. Int Endod J, 55:430-440, 2022. https://doi.org/10.1111/iej.13714
  30. Jang Y, Song M, Yoo IS, Song Y, Roh BD, Kim E : A randomized controlled study of the use of ProRoot Mineral Trioxide Aggregate and Endocem as direct pulp capping materials: 3-month versus 1-year outcomes. J Endod, 41:1201-1206, 2015. https://doi.org/10.1016/j.joen.2015.03.015
  31. Kang CM, Sun Y, Song JS, Pang NS, Roh BD, Lee CY, Shin Y : A randomized controlled trial of various MTA materials for partial pulpotomy in permanent teeth. J Dent, 60:8-13, 2017. https://doi.org/10.1016/j.jdent.2016.07.015
  32. Primus CM, Tay FR, Niu LN : Bioactive tri/dicalcium silicate cements for treatment of pulpal and periapical tissues. Acta Biomater, 96:35-54, 2019. https://doi.org/10.1016/j.actbio.2019.05.050
  33. Kunert M, Lukomska-Szymanska M : Bio-inductive materials in direct and indirect pulp capping - a review article. Materials, 13:1204, 2020.
  34. Kim Y, Lee D, Kye M, Ha YJ, Kim SY : Biocompatible properties and mineralization potential of premixed calcium silicate-based cements and fast-set calcium silicate-based cements on human bone marrow-derived mesenchymal stem cells. Materials, 15:7595, 2022.
  35. Jang YJ, Kim YJ, Vu HT, Park JH, Shin SJ, Dashnyam K, Knowles JC, Lee HH, Jun SK, Han MR, Lee JH, Kim JS, Kim JB, Lee JH, Shin JS : Physicochemical, Biological, and Antibacterial Properties of Four Bioactive Calcium Silicate-Based Cements. Pharmaceutics, 15: 1701, 2023.
  36. Kang TY, Choi JW, Kim KM, Kwon JS : Mechanical and physico-chemical properties of premixed-MTA in contact with three different types of solutions. Korean J Dent Mater, 48:281-292, 2021. https://doi.org/10.14815/kjdm.2021.48.4.281
  37. Coleman NJ, Nicholson JW, Awosanya K : A preliminary investigation of the in vitro bioactivity of white Portland cement. Cem Concr Res, 37:1518-1523, 2007. https://doi.org/10.1016/j.cemconres.2007.08.008
  38. Namazikhah MS, Nekoofar MH, Sheykhrezae MS, Salariyeh S, Hayes SJ, Bryant ST, Mohammadi MM, Dummer PM : The effect of pH on surface hardness and microstructure of mineral trioxide aggregate. Int Endod J, 41:108-116, 2008. https://doi.org/10.1111/j.1365-2591.2007.01325.x
  39. Remadnia A, Dheilly R, Laidoudi B, Queneudec M : Use of animal proteins as foaming agent in cementitious concrete composites manufactured with recycled PET aggregates. Constr Build Mater, 23:3118-3123, 2009. https://doi.org/10.1016/j.conbuildmat.2009.06.027
  40. Jasiczak J, Zielinski K : Effect of protein additive on properties of mortar. Cem Concr Compos, 28:451-457, 2006. https://doi.org/10.1016/j.cemconcomp.2005.12.007
  41. Hesaraki S, Moztarzadeh F, Solati-Hashjin M : Phase evaluation of an effervescent-added apatitic calcium phosphate bone cement. J Biomed Mater Res B Appl Biomater, 79:203-209, 2006. https://doi.org/10.1002/jbm.b.30515
  42. Gemelli E, Lourenci S, Folgueras M, Camargo N : Assessment of industrial wastes in mortar layers deposited on stainless steel sheets of sinks. Ceramica, 50: 336-344, 2004. https://doi.org/10.1590/S0366-69132004000400009
  43. Stutzman P : Scanning electron microscopy imaging of hydraulic cement microstructure. Cem Concr Compos, 26:957-966, 2004. https://doi.org/10.1016/j.cemconcomp.2004.02.043
  44. Ismail MA, Joer HA, Sim WH, Randolph MF : Effect of cement type on shear behavior of cemented calcareous soil. J Geotech Geoenviron Eng, 128:520-529, 2002. https://doi.org/10.1061/(ASCE)1090-0241(2002)128:6(520)
  45. D'Anto V, Di Caprio MP, Ametrano G, Simeone M, Rengo S, Spagnuolo G : Effect of mineral trioxide aggregate on mesenchymal stem cells. J Endod, 36:1839-1843, 2010. https://doi.org/10.1016/j.joen.2010.08.010
  46. Perez AL, Spears R, Gutmann JL, Opperman LA : Osteoblasts and MG-63 osteosarcoma cells behave differently when in contact with ProRoot MTA and White MTA. Int Endod J, 36:564-570, 2003. https://doi.org/10.1046/j.1365-2591.2003.00691.x
  47. Cordeiro MM, Dong Z, Kaneko T, Zhang Z, Miyazawa M, Shi S, Smith AJ, Nor JE : Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth. J Endod, 34:962-969, 2008. https://doi.org/10.1016/j.joen.2008.04.009