Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Extracts of abalone intestine regulates fat metabolism in 3T3-L1 adipocytes and high fat diet-induced zebrafish larvae

  • Laxmi Sen Thakuri (Department of Nutrapharmaceutical Resources, Mokpo National University) ;
  • Chul Min Park (Department of Nutrapharmaceutical Resources, Mokpo National University) ;
  • Jin Yeong Choi (Department of Biomedicine, Health & Life Convergence Sciences, BK21 FOUR, Mokpo National University) ;
  • Hyeon-A Kim (Department of Food and Nutrition, Mokpo National University) ;
  • Han Kyu Lim (Department of Biomedicine, Health & Life Convergence Sciences, BK21 FOUR, Mokpo National University) ;
  • Jin Woo Park (Department of Biomedicine, Health & Life Convergence Sciences, BK21 FOUR, Mokpo National University) ;
  • Dong Wook Kim (Department of Nutrapharmaceutical Resources, Mokpo National University) ;
  • Dong Young Rhyu (Department of Nutrapharmaceutical Resources, Mokpo National University)
  • Received : 2023.12.03
  • Accepted : 2024.01.24
  • Published : 2024.02.29
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Abstract

Obesity is a disease involving mechanisms of fat accumulation, low-grade inflammatory cytokine release, and mitochondrial dysfunction. The aim of the current study was to investigate the effects of abalone intestine extract on fat metabolism in 3T3-L1 adipocytes and high fat diet-induced zebrafish larvae. The total phenol content was highest in subcritical water extract at 210℃ (SW210) among hot water, ethanol, and subcritical water extracts of abalone intestine. In addition, SW210 of male abalone intestine (MASW210) most effectively controlled the lipid accumulation and expression of adipogenic or lipogenic regulators (PPAR-γ, C/EBPα, SREBP-1c, and FAS) in 3T3-L1 adipocytes. Likewise, in zebrafish larvae fed high fat, MASW210 significantly suppressed body weight, glucose levels, and lipid accumulation. The mRNA expression related to adipogenesis (PPAR-γ and C/EBPα), lipogenesis (SREBP-1c and FAS), inflammatory cytokines (TNF-α and IL-6), energy m/;.etabolism (AMPK, lepr, SIRT1, and adiponectin), and mitochondrial biogenesis (PGC-1α and CPT-1) were significantly regulated by treatment with MASW210. These results suggest that abalone intestine extract such as MASW210, are useful biomaterials for improving obesity and metabolic diseases.

Keywords

Acknowledgement

This work was supported by Research Funds of Sabbatical Year of Mokpo National University in 2022.

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