Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Decreased GCF DEL-1 and increased GCF neutrophils with increasing probing pocket depth

  • Seong-Ho Jin (Department of Dentistry, Graduate School, The Catholic University of Korea) ;
  • Eun-Mi Lee (Department of Periodontics, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea) ;
  • Jun-Beom Park (Department of Dentistry, Graduate School, The Catholic University of Korea) ;
  • Youngkyung Ko (Department of Dentistry, Graduate School, The Catholic University of Korea)
  • Received : 2022.12.15
  • Accepted : 2023.06.04
  • Published : 2024.04.30
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Abstract

Purpose: Developmental endothelial locus-1 (DEL-1) plays a role in regulating neutrophil migration within the periodontium. The objective of this study was to evaluate the levels of DEL-1 in saliva and gingival crevicular fluid (GCF), as well as the number of neutrophils in patients with periodontitis. Methods: Forty systemically healthy, non-smoking periodontitis patients participated in this study. Clinical periodontal parameters, including the plaque index, probing pocket depth (PPD), clinical attachment level, bleeding on probing, modified sulcular bleeding index, and marginal bone level, were measured. Levels of DEL-1, interleukin (IL)-1β, IL-6, and IL-8 in unstimulated saliva samples, as well as DEL-1 in the GCF of 3 teeth from each participant, were assessed. Neutrophil counts in oral rinse and GCF samples were recorded. Spearman correlation coefficients were used to examine the correlation between protein levels, clinical parameters, and neutrophil quantities. Participants were divided into 2 age groups (those under 50 years and those 50 years or older) in order to investigate potential age-related differences. Results: DEL-1 levels in the GCF showed a negative relationship with PPD (sum). Neutrophils in oral rinse samples were positively correlated with PPD, IL-8, and IL-1β levels. Neutrophils in GCF exhibited a positive correlation with PPD (sum). Salivary DEL-1 levels showed correlations with IL-8 and IL-1β, but not with the clinical parameters of periodontitis. Conclusions: The negative relationship observed between PPD and GCF DEL-1 levels is consistent with the proposed protective role of DEL-1.

Keywords

Acknowledgement

This research was funded by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, grant number 2018R1D1A1A02043024.

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