Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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Enhancing the Antibacterial Effect of Erythrosine-Mediated Photodynamic Therapy with Ethylenediamine Tetraacetic Acid

  • MinKi Choi (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Haeni Kim (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Siyoung Lee (Department of Microbiology and Immunology, College of Dentistry, Gangneung-Wonju National University) ;
  • Juhyun Lee (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
  • Received : 2023.11.01
  • Accepted : 2023.11.13
  • Published : 2024.02.29
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Abstract

This study evaluated the additive impact of ethylenediamine tetraacetic acid (EDTA) on erythrosine-mediated photodynamic therapy (PDT) against Streptococcus mutans (S. mutans) biofilm by measuring colony-forming units and applying confocal laser scanning microscopy. Fifty-six bovine incisors, free from dental caries or structural defects, were utilized in this study. Dentin specimens were created by cutting with a low-speed diamond disk under a continuous flow of water, resulting in dimensions of 6.0 mm × 3.0 mm × 2.0 mm. The specimens were categorized into 4 groups: Control, EDTA, PDT, and EDTA + PDT. S. mutans ATCC 25175 was employed to establish biofilm on the dentin specimens. A 17% EDTA solution was applied for 1 min. For PDT, erythrosine served as the photosensitizer. Finally, a light-emitting diode source (385 - 515 nm) was employed in this study. The PDT group exhibited a significantly lower bacterial count than both the control and EDTA groups (p < 0.001). The EDTA + PDT group demonstrated a significantly reduced bacterial count compared to the other 3 groups (p < 0.001). This study demonstrated that EDTA enhances the antimicrobial efficacy of PDT on S. mutans biofilm. Even at a low concentration of photosensitizer, the combination of EDTA and PDT yields a significant antibacterial effect.

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References

  1. Selwitz RH, Ismail AI, Pitts NB : Dental caries. Lancet, 369:51-59, 2007. 
  2. Kazeminia M, Abdi A, Shohaimi S, Jalali R, Vaisi-Raygani A, Salari N, Mohammadi M : Dental caries in primary and permanent teeth in children's worldwide, 1995 to 2019: a systematic review and meta-analysis. Head Face Med, 16:22, 2020. 
  3. Islam B, Kahn SN, Kahn AU : Dental caries: from infection to prevention. Med Sci Monit, 13:RA196-203, 2007. 
  4. Konopka K, Goslinski T : Photodynamic therapy in dentistry. J Dent Res, 86:694-707, 2007. 
  5. Kim M, Jung HY, Park HJ : Topical PDT in the treatment of benign skin disease: Principles and new applications. Int J Mol Sci, 16:23259-23278, 2015. 
  6. da Mota, ACC, Leal CRL, Olivan S, Goncalves MLL, de Oliveira VA, Pinto MM, Bussadori SK : Case report of photodynamic therapy in the treatment of dental caries on primary teeth. J Lasers Med Sci, 7:131-133, 2016. 
  7. Park D, Choi EJ, Weon KY, Lee W, Lee SH, Choi JS, Park GH, Lee B, Byun MR, Baek K, Choi JW : Non-invasive photodynamic therapy against - periodontitis-causing bacteria. Sci Rep, 9:8248, 2019. 
  8. Saini R, Lee NV, Liu KYP, Poh CF : Prospects in the application of photodynamic therapy in oral cancer and premalignant lesions. Cancers (Basel), 8:83, 2016. 
  9. Nagata JY, Hioka N, Kimura E, Batistela VR, Terada RSS, Graciano AX, Baesso ML, Hayacibara MF : Antibacterial photodynamic therapy for dental caries: Evaluation of the photosensitizers used and light source properties. Photodiagnosis Photodyn Ther, 9:122-131, 2012. 
  10. Costa ACBP, Rasteiro VMC, Pereira CA, Rossoni RD, Junqueira JC, Jorge AOC : The effects of rose bengaland erythrosine- mediated photodynamic therapy on Candida albicans. Mycoses, 55:56-63, 2012. 
  11. Wood S, Metcalf D, Devine D, Robinson C : Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms. J Antimicrob Chemother, 57:680-684, 2006. 
  12. Mohammadi Z, Shalavi S, Jafarzadeh H : Ethylenediaminetetraacetic acid in endodontics. Eur J Dent, 7(Suppl 1):S135-S142, 2013. 
  13. von der Fehr FR, Nygaard-Ostby B : Effect of EDTAC and sulfuric acid on root canal dentine. Oral Surg Oral Med Oral Pathol, 16:199-205, 1963. 
  14. Leive L : Release of lipopolysaccharide by EDTA treatment of E. coli. Biochem Biophys Res Commun, 21:290-296, 1965. 
  15. Lee YH, Park HW, Lee JH, Seo HW, Lee SY : The photodynamic therapy on Streptococcus mutans biofilms using erythrosine and dental halogen curing unit. Int J Oral Sci, 4:196-201, 2012. 
  16. Jung JS, Park HW, Lee JH, Seo HW, Lee SY : The effect of photodynamic therapy on the viability of Streptococcus mutans isolated from oral cavity. J Korean Acad Pediatr Dent, 39:233-241, 2012. 
  17. Kim JY, Park HW, Lee JH, Seo HW, Lee SY : Antimicrobial effect on Streptococcus mutans in photodynamic therapy using different light source. J Korean Acad Pediatr Dent, 45:82-89, 2018. 
  18. Fernandes FGL, de Moraes FB, de Cezare JA, Degasperi GR, Fontana CE, Grandizoli DRP, Pinheiro SL : In vitro evaluation of EDTA combined with photodynamic therapy to reduce Streptococcus mutans in carious dentin. Photodiagnosis Photodyn Ther, 37:102718, 2022. 
  19. Ho T, Guidolin K, Makky A, Valic M, Ding L, Bu J, Zheng M, Cheng MHY, Yau J, Chen J, Zheng G : Novel strategy to drive the intracellular uptake of lipid nanoparticles for photodynamic therapy. Angew Chem Int Ed Engl, 62:E202218218, 2023. 
  20. Serper A, Calt S : The demineralizing effects of EDTA at different concentrations and pH. J Endod, 28:501-502, 2002. 
  21. Calt S, Serper A : Time-dependent effects of EDTA on dentin structure. J Endod, 28:17-19, 2002. 
  22. Lee JK, Park SH, Choi GW : Time-dependent effects of EDTA application on removal of smear layer in the root canal system. J Korean Acad Conserv Dent, 31:169-178, 2006. 
  23. Ganesan L, Margolles-Clark E, Song Y, Buchwald P : The food colorant erythrosine is a promiscuous protein-protein interaction inhibitor. Biochem Pharmacol, 81:810-818, 2011. 
  24. Choi SJ, Park HW, Lee JH, Seo HW, Lee SY : Optimum treatment parameters for photodynamic antimicrobial chemotherapy on streptococcus mutans biofilms. J Korean Acad Pediatr Dent, 42:151-157, 2015. 
  25. Chequer FMD, de Paula Venancio V, Bianchi ML, Antunes LMG : Genotoxic and mutagenic effects of erythrosine B, a xanthene food dye, on HepG2 cells. Food Chem Toxicol, 50:3447-3451, 2012. 
  26. Nima G, Soto-Montero J, Alves LA, Mattos-Graner RO, Giannini M : Photodynamic inactivation of Streptococcus mutans by curcumin in combination with EDTA. Dent Mater, 37:E1-E14, 2021. 
  27. Saitawee D, Teerakapong A, Morales NP, Jitprasertwong P, Hormdee D : Photodynamic therapy of curcuma longa extract stimulated with blue light against Aggregatibacter actinomycetemcomitans. Photodiagnosis Photodyn Ther, 22:101-105, 2018. 
  28. Lee JK, Park SH, Lee SY, Chang BS, Um HS : The effect of erythrosine-mediated photodynamic therapy on intraorally formed biofilm on titanium surface. Int J Oral Biol, 37:103-108, 2012. 
  29. Umerska A, Strandh M, Cassisa V, Matougui N, Eveillard M, Saulnier P : Synergistic effect of combinations containing EDTA and the antimicrobial peptide AA230, an arenicin-3 derivative, on gram-negative bacteria. Biomolecules, 8:122, 2018. 
  30. Patterson SS : In vivo and in vitro studies of the effect of the disodium salt of ethylenediamine tetra-acetate on human dentine and its endodontic implications. Oral Surg Oral Med Oral Pathol, 16:83-103, 1963. 
  31. Heling I, Chandler NP : Antimicrobial effect of irrigant combinations within dentinal tubules. Int Endod J, 31:8-14, 1998. 
  32. Park C, Park HW, Lee JH, Seo HW, Lee SY : Application of teeth whitening LED for prevention of dental caries : Antimicrobial photodynamic therapy approach. J Korean Acad Pediatr Dent, 47:70-77, 2020. 
  33. Gong JE, Seo HW, Lee SY, Park HW, Lee JH : Susceptibility of mutans streptococci in the planktonic and biofilm state to erythrosine. J Korean Acad Pediatr Dent, 46:135-138, 2019. 
  34. Garcez AS, Nunez SC, Azambuja N Jr, Fregnani ER, Rodriguez HMH, Hamblin MR, Suzuki H, Ribeiro MS : Effects of photodynamic therapy on gram-positive and gram-negative bacterial biofilms by bioluminescence imaging and scanning electron microscopic analysis. Photomed Laser Surg, 31:519-525, 2013. 
  35. Taweewattanapaisan P, Jantarat J, Ounjai P, Janebodin K : The effects of EDTA on blood clot in regenerative endodontic procedures. J Endod, 45:281-286, 2019. 
  36. Mendez DAC, Rizzato VL, Lamarque GCC, Dionisio EJ, Buzalaf MAR, Rios D, Machado MAAM, Cruvinel T : Could a chelant improve the effect of curcumin-mediated photodynamic antimicrobial chemotherapy against dental intact biofilms? Laser Med Sci, 34:1185-1192, 2019.