DOI QR코드

DOI QR Code

Monitoring Hazards to Verify the Safety of Plant-Based Meat Alternatives

식물성 대체육의 안전성 검증을 위한 위해요소 모니터링

  • Ayeong Ma (Department of Food and Nutrition, Chonnam National Universit) ;
  • Eun Sung Shin (Department of Food and Nutrition, Chonnam National Universit) ;
  • Seon-A Son (Department of Food and Nutrition, Chonnam National Universit) ;
  • Tai-Sun Shin (Department of Food and Nutrition, Chonnam National Universit) ;
  • Hyun-Jung Chung (Department of Food and Nutrition, Chonnam National Universit)
  • Received : 2024.03.13
  • Accepted : 2024.03.30
  • Published : 2024.04.30

Abstract

The proportion of plant-based meat alternative (PBMA) consumers has recently increased in Korea. This is due to several reasons including protecting the environment, satisfying preferences, maintaining health, and improving eating habits. Accordingly, many companies produce and sell alternative meat using various materials. Alternative meats are classified into plant (such as soybeans and wheat), seaweed, insect, and cultured meats, depending on the raw materials used in manufacturing. PBMA is sold after undergoing processes such as grinding, seasoning, and molding. Therefore, monitoring the presence of any hazardous elements during this process is essential. Accordingly, in this study, we analyzed the harmful components of nine domestically distributed PBMA that are most easily accessible to consumers. After extracting fat from the samples and analyzing the rancidity level, samples F, G, and I were highly rancid. Trace amounts of aflatoxin were detected in samples A and B, but confirmed to be within the range. Cd and Pb were not detected in any sample. We hope that this study will help establish methods to ensure the safety of domestically sold PBMA.

국내에서 제조되어 유통되는 식물성 대체육을 대상으로 일반성분(수분, 단백질, 지방), 지방의 산패도, 지방산의 조성, 아플라톡신, 중금속 함량을 분석하였다. 그 결과 한번 익힌 후 건조된 형태의 식물성 대체육보다 익히지 않고 수분이나 지방에 의해 성형과정을 거친 냉동제품에서 상대적으로 많은 양의 지방을 함유하고 있음을 확인할 수 있었다. 조지방 함량이 1 g을 넘는 시료를 대상으로 지방의 산패도(산가, 과산화물가)를 측정했다. 그 결과 일부 시료에서 높은 산가 및 과산화물가가 측정되었으나 식품공전상에 대체육에 대한 정확한 분류가 되어있지 않아 산패도에 대한 안전성을 판단하기 위한 규격이 필요해 보인다. 가스크로마토그래피를 사용하여 지방산 조성을 분석한 결과 대부분은 불포화지방산이 차지하고 있지만, 일부 포화지방산의 함량이 높은 시료가 있었다. 포화지방산 및 불포화지방산의 함량이 높은 식물성 대체육을 식물성 유지를 사용하여 가열조리를 거쳐 섭취하게 된다면, 많은 양의 지방을 섭취하게 될 수 있음을 예측할 수 있다. 또 가스크로마토그래피를 사용한 지방산 분석법을 통해 식물성대체육에 동물 유래 지질이 함유되어있지 않음을 확인할 수 있었다. 이는 이후에 식물성 대체육에 대한 영양표시성분 및 실제성분의 대조 시에 활용될 수 있음을 시사한다. 식물성 대체육에 대하여 아플라톡신을 분석한 결과 현재 식품공전에 제시된 농산물 중 아플라톡신 기준규격에 대하여 적합인 수준이나 미량 검출되는 것을 확인하였다. 중금속의 분석 결과 모두 불검출이었으나 환경요인 및 원재료에 따라 중금속의 오염이 의심될 시에 중금속 분석을 진행할 수 있을 것으로 판단된다. 이러한 실험결과를 통해 국내에 유통되고 있는 일부 식물성 대체육에 대한 안전성을 확인하였으나 산가, 과산화물가, 아플라톡신, 중금속은 대체육에 대하여 구체적인 분류 및 적합 규격이 마련되어 있지 않아 적합 판정시에 비슷한 성향을 가진 식품군에 대조하여 판단해야 하는 어려움이 있었다. 따라서 기존의 기준에 대하여 검토 또는 변경을 거쳐 식물성대체육에 대한 관리기준이 설정한다면 유사한 식품군의 건전성 및 적합성을 유지하는 것에 도움이 될 것으로 판단된다.

Keywords

References

  1. Tziva, M., Negro, S.O., Kalfagianni, A., Hekkert, M.P., Understanding the protein transition: the rise of plant-based meat substitutes. Environ. Innov. Soc. Trans., 35, 217-231 (2020). https://doi.org/10.1016/j.eist.2019.09.004
  2. Derbyshire, E.J., Flexitarian diets and health: a review of the evidence-based literature. Front. Nutr., 3, 1-8 (2017). https://doi.org/10.3389/fnut.2016.00055
  3. Andreani, G., Sogari, G., Marti, A., Froldi, F., Dagevos, H., Martini, D., Plant-based meat alternatives: technological, nutritional, environmental, market, and social challenges and opportunities. Nutrients, 15, 12-16 (2023). https://doi.org/10.3390/nu15020452
  4. Grosso, G., La Vignera, S., Condorelli, R.A., Godos, J., Marventano, S., Tieri, M., Ghelfi, F., Titta, L., Lafranconi, A., Gambera, A., Alonzo, E., Sciacca, S., Buscemi, S., Ray, S., Rio, D.D., Galvano, F., Total, red and processed meat consumption and human health: an umbrella review of observational studies. Int. J. Food Sci. Nutr., 73, 726-737 (2022). https://doi.org/10.1080/09637486.2022.2050996
  5. Onwezen, M.C., Verain, M.C.D., Dagevos, H., social norms support the protein transition: the relevance of social norms to explain increased acceptance of alternative protein burgers over 5 years. Foods, 11, 3413 (2022).
  6. Ahmad, M., Qureshi, S., Akbar, M.H., Siddiqui, S.A., Gani, A., Mushtaq, M., Hassana, I,, Dhull, S.B., Plant-based meat alternatives: Compositional analysis, current development and challenges. Appl. Food Res., 2, 100154 (2022).
  7. Haque, M.A., Timilsena, Y.P., Adhikari, B., Food proteins, structure, and function. Food Sci., 163-166 (2016).
  8. De Marchi, M., Costa, A., Pozza, M., Goi, A., Manuelian, C.L., Detailed characterization of plant-based burgers. Sci. Rep., 11, 1-9 (2021). https://doi.org/10.1038/s41598-020-79139-8
  9. Pointke, M., Pawelzik, E., Plant-based alternative products: are they healthy alternatives? micro- and macronutrients and nutritional scoring. Nutrients, 14, 601 (2022).
  10. Gorissen, S.H.M., Crombag, J.J.R., Senden, J.M.G., Waterval, W.A.H., Bierau, J., Verdijk, L.B., van Loon, L.J.C., Protein content and amino acid composition of commercially available plant-based protein isolates. Amino Acids, 50, 1685-1695 (2018). https://doi.org/10.1007/s00726-018-2640-5
  11. Fiorentini, M., Kinchla, A.J., Nolden, A.A., Role of sensory evaluation in consumer acceptance of plant-based meat analogs and meat extenders: a scoping review. Foods, 9, 1334 (2020).
  12. Zahari, I., Ostbring, K., Purhagen, J.K., Rayner, M., Plant-based meat analogues from alternative protein: a systematic literature review. Foods, 11, 2870 (2022).
  13. Ministry of Food and Drug Safety (MFDS), (2024, January 24). Korea food code. Retrieved from https://various.foodsafetykorea.go.kr/fsd/#/ext/Document/FC
  14. Griffiths, M.J., Vanhille, R.P., Harrison, S.T.L., Selection of direct transesterification as the preferred method for assay of fatty acid content of microalgae. Lipids, 45, 1053-1060 (2010). https://doi.org/10.1007/s11745-010-3468-2
  15. Kim, K., Choi, B., Lee, I., Lee, H., Kwon, S., Oh, K., Kim, A.Y., Bioproduction of mushroom mycelium of Agaricus bisporus by commercial submerged fermentation for the production of meat analogue. J. Sci. Food Agric., 91, 1561-1568 (2011). https://doi.org/10.1002/jsfa.4348
  16. Kumar, P., Chatli, M.K., Mehta, N., Singh, P., Malav, O.P., Verma, A.K., Meat analogues: Health promising sustainable meat substitutes. Crit. Rev. Food Sci. Nutr., 57, 923-932 (2017). https://doi.org/10.1080/10408398.2014.939739
  17. Ooms, N., Jansens, K.J.A., Pareyt, B., Reyniers, S., Brijs, K., Delcour, J.A., The impact of disulfide bond dynamics in wheat gluten protein on the development of fermented pastry crumb. Food Chem., 242, 68-74 (2018). https://doi.org/10.1016/j.foodchem.2017.09.007
  18. Chiang, J.H., Tay, W., Ong, D.S.M., Liebl, D., Ng, C.P., Henry, C.J., Physicochemical, textural and structural characteristics of wheat gluten-soy protein composited meat analogues prepared with the mechanical elongation method. Food Struct., 28, 100183 (2021).
  19. Yi, B.R., Kim, M.J., Lee, J.H., Lipid oxidation and antioxidant mechanisms in different matrix. Food Sci. Ind., 51, 127-135 (2018).
  20. Chae, S.K., Kim, S.H., Shin, D.H., Oh, H.K., Lee, S.J., Jang, M.H., Choi, W., (2005). Food chemistry, 3rd ed, Hyoil Books Publishing, Seoul, Korea, pp. 147-164.
  21. Sa, D.C., Jeon, S,H., Jeon, H.J., Suggestions for the management of cultured meat under the food sanitation act. Jhlr, 23, 349-366 (2022).
  22. Jeon, J.Y., Jeong, M.J., Nam, J.W., Nam, S.Y., Kim, B.M., Inhibitory effect of an antioxidant complex on the lipid oxidation of freeze-dried convenience food. Food Sci. Preserv., 28, 654-662 (2021). https://doi.org/10.11002/kjfp.2021.28.5.654
  23. Geerts, M.E.J., Dekkers, B.L., van der Padt, A., van der Goot, A.J., Aqueous fractionation processes of soy protein for fibrous structure formation. Innov. Food Sci. Emerg., 45, 313-319 (2018). https://doi.org/10.1016/j.ifset.2017.12.002
  24. Hu, F.B., Otis, B.O., McCarthy, G., Can plant-based meat alternatives be part of a healthy and sustainable diet? JAMA, 322, 1547-1548 (2019). https://doi.org/10.1001/jama.2019.13187
  25. Kim, S.D., Kim, A.K., Lee, H.K., Lee, S.L., Lee, H.J., Ryu, H.J., Lee, J.M., Yu, I.S., Jung, K., A monitoring of aflatoxins in commercial herbs for food and medicine. J. Food Hyg. Saf., 32, 267-274 (2017). https://doi.org/10.13103/JFHS.2017.32.4.267
  26. Gourama, H., Bullerman, L.B., Aspergillus flavus and Aspergillus parasiticus; Aflatoxigenic fungi of concern in foods and feeds-A review. J. Food Prot., 58, 1395-1404 (1995). https://doi.org/10.4315/0362-028X-58.12.1395
  27. International Agency for Research on Cancer (IARC), Some naturally occurring substances : food items and constituents, hetrocyclic aromatic amines and mycotoxins. World Health Organization (WHO), 56, 245-395 (1993).
  28. Lee, Y.W., Kim, J.C., Natural occurrence of fusarium mycotoxins in cereals. J. Food Hyg. Saf., 8, 23-32 (1993).
  29. Manzoor, J., Sharma, M., Wani, K.A., Heavy metals in vegetables and their impact on the nutrient quality of vegetables: A review. J. Plant Nutr., 41, 1744-1763 (2018). https://doi.org/10.1080/01904167.2018.1462382
  30. Kim, H.Y., Kim, J.I., Kim, J.C., Park, J.E., Lee, K.J., Kim, S.I., Oh, J.H., Jang, Y.M., Survey of heavy metal contents of circulating agricultural products in Korea. Korean J. Food Sci. Technol., 41, 238-244 (2009).