Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Optimization and Applicability Verification of Simultaneous Chlorogenic acid and Caffeine Analysis in Health Functional Foods using HPLC-UVD

HPLC-UVD를 이용한 건강기능식품에서 클로로겐산과 카페인 동시분석법 최적화 및 적용성 검증

  • Hee-Sun Jeong (Nutrition and Functional Food Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Se-Yun Lee (Nutrition and Functional Food Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Kyu-Heon Kim (Nutrition and Functional Food Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Mi-Young Lee (Nutrition and Functional Food Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Jung-Ho Choi (Nutrition and Functional Food Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Jeong-Sun Ahn (Nutrition and Functional Food Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Jae-Myoung Oh (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Kwang-Il Kwon (Nutrition and Functional Food Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Hye-Young Lee (Nutrition and Functional Food Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
  • 정희선 (식품의약품안전처 식품의약품안전평가원 영양기능연구과) ;
  • 이세윤 (식품의약품안전처 식품의약품안전평가원 영양기능연구과) ;
  • 김규헌 (식품의약품안전처 식품의약품안전평가원 영양기능연구과) ;
  • 이미영 (식품의약품안전처 식품의약품안전평가원 영양기능연구과) ;
  • 최정호 (식품의약품안전처 식품의약품안전평가원 영양기능연구과) ;
  • 안정선 (식품의약품안전처 식품의약품안전평가원 영양기능연구과) ;
  • 오재명 (식품의약품안전처 식품의약품안전평가원 첨가물포장과) ;
  • 권광일 (식품의약품안전처 식품의약품안전평가원 영양기능연구과) ;
  • 이혜영 (식품의약품안전처 식품의약품안전평가원 영양기능연구과)
  • Received : 2023.11.02
  • Accepted : 2024.04.18
  • Published : 2024.04.30
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Abstract

In this study, we analyzed chlorogenic acid indicator components in preparation for the additional listing of green coffee bean extract in the Health Functional Food Code and optimized caffeine for simultaneous analysis. We extracted chlorogenic acid and caffeine using 30% methanol, phosphoric acid solution, and acetonitrile-containing phosphoric acid and analyzed them at 330 and 280 nm, respectively, using liquid chromatography. Our analysis validation results yielded a correlation coefficient (R2) revealing a significance level of at least 0.999 within the linear quantitative range. The chlorogenic acid and caffeine detection and quantification limits were 0.5 and 0.2 ㎍/mL and 1.4, and 0.4 ㎍/mL, respectively. We confirmed that the precision and accuracy results were suitable using the AOAC validation guidelines. Finally, we developed a simultaneous chlorogenic acid and caffeine analysis approach. In addition, we confirmed that our analysis approach could simultaneously quantify chlorogenic acid and caffeine by examining the applicability of each formulation through prototypes and distribution products. In conclusion, the results of this study demonstrated that the standardized analysis would expectably increase chlorogenic acidcontaining health functional food quality control reliability.

본 연구는 그린커피빈추출물이 「건강기능식품의 기준 및 규격」에 추가로 등재될 경우를 대비하여 표준화된 클로로겐산 시험법을 설정하고, 카페인이 동시 분석되도록 최적화하는 연구를 진행하였다. 최적화된 시험법을 마련하기 위해 기기분석 및 전처리 조건을 비교·분석하여 클로로겐산과 카페인을 30% 메탄올 추출하여 인산용액과 인산 함유 아세토니트릴으로 액체크로마토그래프를 통해 330 nm, 280 nm에서 분석하도록 시험법을 설정하였다. 시험법 밸리데이션 결과, 직선성 정량범위 내에서 상관계수(R2) 0.999 이상의 유의수준을 보였고, 클로로겐산과 카페인 검출한계는 0.5와 0.2 ㎍/mL, 정량한계는 1.4와 0.4 ㎍/mL로 나타났다. 정밀도와 정확도 결과는 AOAC 밸리데이션 가이드라인를 통해 적합함을 확인하였고, 클로로겐산 및 카페인 동시분석법을 최종적으로 마련하였다. 또한, 시제품과 유통제품을 통해 제형별 적용성 검토하여 클로로겐산과 카페인을 동시에 정량 가능한 시험법임을 재확인하였다. 최적화된 시험법은 클로로겐산을 함유한 건강기능식품 품질관리에 대한 신뢰성을 더 높일 것으로 본다.

Keywords

Acknowledgement

본 연구는 2021년도 식품의약품안전처 연구개발사업의 연구비지원(21161미래식044)에 의해 수행되었으며, 이에 감사드립니다.

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