한국환경보건학회지 (Journal of Environmental Health Sciences)

  • 제50권2호
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  • Pages.93-101
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  • 2024
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  • 1738-4087(pISSN)
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  • 2233-8616(eISSN)
한국환경보건학회 (Korean Society of Environmental Health)
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광주지역 공공수역의 미생물 군집 다양성 및 항생제 내성에 관한 연구

A Study on Microbial Community Diversity and Antibiotic Resistance in Public Waters in Gwangju

  • 김선정 (광주광역시 보건환경연구원) ;
  • 박지영 (광주광역시 보건환경연구원) ;
  • 김승호 (광주광역시 보건환경연구원) ;
  • 임민화 (광주광역시 보건환경연구원) ;
  • 유지용 (광주광역시 보건환경연구원) ;
  • 한규성 (광주광역시 보건환경연구원) ;
  • 박세일 (광주광역시 보건환경연구원) ;
  • 서광엽 (광주광역시 보건환경연구원) ;
  • 조광운 (광주광역시 보건환경연구원)
  • Sun-Jung Kim (Health and Environment Research Institute of Gwangju) ;
  • Ji-Young Park (Health and Environment Research Institute of Gwangju) ;
  • Seung-Ho Kim (Health and Environment Research Institute of Gwangju) ;
  • Min-Hwa Lim (Health and Environment Research Institute of Gwangju) ;
  • Ji-Yong Yu (Health and Environment Research Institute of Gwangju) ;
  • Kyu-Sung Han (Health and Environment Research Institute of Gwangju) ;
  • Se-Il Park (Health and Environment Research Institute of Gwangju) ;
  • Gwangyeob Seo (Health and Environment Research Institute of Gwangju) ;
  • Gwangwoon Cho (Health and Environment Research Institute of Gwangju)
  • 투고 : 2024.02.27
  • 심사 : 2024.03.27
  • 발행 : 2024.04.30
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초록

Background: As pollutants caused by non-point sources flow into rivers, river water quality monitoring for fecal pollution is becoming increasingly important. Objectives: This study was conducted to investigate the distribution of microbial communities in the Yeongsangang River water system and sewage treatment plants in Gwangju and to evaluate their antibiotic resistance. Methods: In the experiment, samples were distributed to five selective media at each point and then cultured for 18 to 24 hours. When bacteria were observed, they were sub-cultured by size and shape and identified using MALDI-TOF MS equipment. When identification was completed, 17 types of antibiotic susceptibility tests were performed using VITEK II equipment, focusing on gram-negative dominant species among the identified strains. Results: During the study period, a total of 266 strains were isolated from 39 samples. Gram-positive bacteria were 37 strains in four genera, or 13.9% of the total, and Gram-negative bacteria were 229 strains in 23 genera, or 86.1% of the total. Antibiotic susceptibility testing of 23 strains, the major dominant species, showed that one strain (4.3%) was resistant to only one antibiotic, and two strains (8.7%) were 100% susceptible to the 17 antibiotics tested. The other 20 strains (87.0%) were multidrug resistant bacteria resistant to two or more antibiotics. There were various types of multidrug resistance. Among them, penicillin and cephalosporin series showed the highest resistance. Conclusions: Based on the results of this study, it was found that the bacterial community structure changed according to regional and environmental factors, and it was judged that continuous research such as genetic analysis of antibiotic-resistant bacteria present in natural rivers is necessary.

키워드

과제정보

이 연구는 2023년 환경부의 「환경분야 시험검사의 국제적 적합성 기반구축사업」재원으로 국립환경과학원의 지원(2023-01-03-002)과 광주광역시 보건환경연구원의 「연구지원 및 역량강화」의 일환으로 수행하였습니다.

참고문헌

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