한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제37권1호
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  • Pages.79-86
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  • 2024
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  • 1226-3591(pISSN)
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  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
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변온 조건 및 GA3 처리가 큰금매화의 종자 발아에 미치는 영향

Effect of Alternating Temperature Conditions, and GA3 Treatment on Seed Germination of Trollius macropetalus (Regel) F.Schmidt

  • 김동학 (국립수목원, DMZ산림생물자원보전과) ;
  • 조승주 (강원대학교, 산림환경시스템학과) ;
  • 성정원 (한국농수산대학교, 조경학과) ;
  • 윤정원 (국립수목원, DMZ산림생물자원보전과)
  • Dong-Hak Kim (DMZ Botanic Garden, Korea National Arboretum) ;
  • Seungju Jo (Department of Forest Environmental System, Kangwon National University) ;
  • Jung-Won Sung (Department of Landscape Architecture, Korea National University of Agriculture and Fisheries) ;
  • Jung-Won Yoon (DMZ Botanic Garden, Korea National Arboretum)
  • 투고 : 2023.09.06
  • 심사 : 2023.12.11
  • 발행 : 2024.02.01
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초록

본 연구는 북한 식물자원 큰금매화의 종자 번식을 위해 발아에 대한 기초자료를 제시하고자 수행되었다. 큰금매화 종자의 형태적 특성과 활력을 검정하였고, 변온 조건(15/6℃ & 25/15℃), 저온층적처리 및 GA3 처리에 따른 발아 특성을 조사하였다. 연구 결과, 저온층적처리는 큰금매화 종자의 휴면타파에 효과가 없었다. GA3 처리는 큰금매화 종자의 발아율을 현저히 증가시켰고, 평균발아일수와 발아세 또한 유의미하게 향상시켰다. 한편, 15/6℃ 조건에 비해 25/15℃ 조건에서는 큰금매화 종자에 대한 GA3 처리의 효과가 감소하였다. 큰금매화 종자는 Nondeep-type의 형태·생리적휴면(MPD)을 가지는 것으로 판단되었고, 본 연구에서 가장 효율적인 발아 조건은 15/6℃ 변온조건과 GA3 500 mg·L-1 처리로 확인되었다. 이러한 본 연구의 결과는 고산성 북방계 식물인 큰금매화의 대량증식에 유용한 자료가 될 것이다.

This study was conducted to determine the conditions for breaking dormancy in the seeds of the North Korean plant resource, Trollius macropetalus, and to investigate its germination characteristics. The morphological characteristics and viability of T. macropetalus seeds were examined. Germination characteristics were investigated based on temperature conditions (15/6℃ & 25/15℃), cold stratification treatment, and GA3 treatment. The results showed that cold stratification treatment had no effect on breaking the dormancy of T. macropetalus. GA3 treatment significantly increased the germination rate of T. macropetalus and also improved the mean germination days and germination speed. However, compared to the 15/6℃ condition, the effectiveness of GA3 treatment for T. macropetalus decreased under the 25/15℃ condition. It was determined that T. macropetalus have a nondeep-type morpho-physiological dormancy. The most effective conditions for breaking dormancy were found to be the 15/6℃ temperature condition and GA3 treatment at 500 mg·L-1. The findings of this study are expected to provide valuable information for the mass propagation of T. macropetalus, which has potential applications in landscaping and pharmaceutical materials.

키워드

참고문헌

  1. Baskin, C.C. and J.M. Baskin. 1998. Seeds: ecology, biogeography, and evolution of dormancy and germination. Academic Press, San Diago, CA (USA).
  2. Baskin, C.C. and J.M. Baskin. 2014. Seeds: ecology, biogeography, and evolution of dormancy and germination, 2nd edn. Academic Press, San Diago, CA (USA).
  3. Baskin, J.M. and C.C. Baskin. 2004. A classification system for seed dormancy. Seed Sci. Res. 14(1):1-16.
  4. Cho, J.S., B.K. Jang and C.H. Lee. 2018. Seed dormancy and germination characteristics of the endangered species Cicuta virosa L. in South Korea. Hortic Environ Biotechnol. 59:473-481. https://doi.org/10.1007/s13580-018-0062-7
  5. DPR of Korea. 2007. National biodiversity strategy and action plan for DPR Korea. DPR of Korea, Pyongyang, DPR of Korea (in Korean).
  6. Ellis, R.A. 1981. The quantification of agent and survival in orthodox seeds. Seed Sci. Technol. 9:373-409.
  7. Finch-Savage, W.E. and G. Leubner-Metzger. 2006. Seed dormancy and the control of germination. New Phytol. 171:501-523. https://doi.org/10.1111/j.1469-8137.2006.01787.x
  8. Gantsetseg, A., S.Y. Jung, W.B. Cho, E.K. Han, S.K. So and J.H. Lee. 2020. Definition and species list of northern lineage plants on the Korean Peninsula. Korean Herb. Med. Inf. 8(2):183-204 (in Korean).
  9. Geneve, R.L. 2003. Impact of temperature on seed dormancy. Hort. Sci. 38(3):336-340.
  10. Gusar, A.S., L.V. Buglova and M.V. Kozlova. 2021. The estimation of Trollius L. species and hybrids by seed germination. Probl. bot. Ûzn. Sib. Mong. 20(1):147-150.
  11. Hay, F.R. and R.J. Probert. 2013. Advances in seed conservation of wild plant species: a review of recent research. Conserv. Physiol. 1(1):cot030.
  12. Hepher, A. and J.A. Roberts. 1985. The control of seed germination in Trollius ledebouri a model of seed dormancy. Planta 166:321-328. https://doi.org/10.1007/BF00401168
  13. International Seed Testing Association (ISTA). 2014. International Rules for Seed Testing, Chapter 6: The Tetrazolium Test. ISTA, Bassersdorf, Switzerland.
  14. Jang, B.K., J.S. Cho and C.H. Lee. 2016. Effect of environmental conditions and chemical treatments on seed germination of Astilbe koreana (Kom.) Nakai. Korean J. Plant Res. 29(2):235-240 (in Korean). https://doi.org/10.7732/kjpr.2016.29.2.235
  15. Jiang, M., L. Yan, K.A. Li, Z.H. Ji and S.G. Tian. 2020. Evaluation of total phenol and flavonoid content and antimicrobial and antibiofilm activities of Trollius chinensis Bunge extracts on Streptococcus mutans. Microsc. Res. Tech. 83(12):1471-1479. https://doi.org/10.1002/jemt.23540
  16. Kim, D.H., Y.E. Kim, S. Jo, J.W. Lee and S.J. Kim. 2023. Effect of temperature conditions and chemical treatments on seed germination of Pseudolysimachion kiusianum var. diamantiacum (Nakai) T.Yamaz. Korean J. Plant Res. 36(4):381-389 (in Korean).
  17. Kim, Y.H. and I.J. Lee. 2013. Influence of plant growth regulator application on seed germination of dandelion (Taraxacum officinale). Weed Turf. Sci. 2(2):152-158 (in Korean). https://doi.org/10.5660/WTS.2013.2.2.152
  18. Lee, H.S., J.E. Jang, D.L. Yoo and S.Y. Ryu. 2003. Effects of temperature and gibberellin treatments on seed germination of Megaleranthis saniculifolia. J. Kor. Soc. Hort. Sci. 44(3):388-392 (in Korean).
  19. Lee, T.B. 2003. Coloured Flora of Korea, Vol I. Hayangmunsa, Seoul, Korea (in Korean).
  20. Li, Y.L., S.C. Ma, Y.T. Yang, S.M. Ye and P.P.H. But. 2002. Antiviral activities of flavonoids and organic acid from Trollius chinensis Bunge. J. Ethnopharmacol. 79(3):365-368. https://doi.org/10.1016/S0378-8741(01)00410-X
  21. Lu, J., P.Z. Qin, X. Han, Y.P. Wang and Z.H. Li. 2015. Evaluation of antioxidant and antibacterial properties of extracts from Trollius chinensis Bunge. Eur. Food Res. Technol. 240:301-310. https://doi.org/10.1007/s00217-014-2329-2
  22. Luferov, A.N., A.S. Erst, D.N. Luferov, A.I. Shmakov and W. Wang. 2018. The genus Trollius (Ranunculaceae) in the Russian Far East. Turczaninowia 21(2):110-116.
  23. Martin, A.C. 1946. The comparative internal morphology of seeds. Am. Midl. Nat. 36(3):513-660.
  24. Nonogaki, H. 2019. Seed germination and dormancy: the classic story, new puzzles, and evolution. J. Integr. Plant Biol. 61:541-563. https://doi.org/10.1111/jipb.12762
  25. O'Donnell, K. and S. Sharrock. 2017. The contribution of botanic gardens to ex situ conservation through seed banking. Plant Divers. 39(6):373-378. https://doi.org/10.1016/j.pld.2017.11.005
  26. Park, C.H. and J.S. Yoo. 2009. Investigation of forest degradation of North Korea based on remote sensing. J. Environ. Sci. 48:3-24 (in Korean).
  27. Park, C.W. and H.W. Lee. 2018. Ranunculaceae, in the genera of vascular plants of Korea. In Flora of Korea Editorial Committee (ed.), Hongreung Publishing Co., Seoul, Korea (in Korean).
  28. Park, H.B., C.H. Ko, S.Y. Kim, K.C. Lee, J.H. Kim and J.M. Chung. 2019. Dormancy type and germination characteristics of seeds of Lonicera chrytsantha Turcz. Ex Ledeb (Caprifoliaceae). Korean J. Plant Res. 32(5):457-462 (in Korean).
  29. Serebryanyi, M.M. 2019. Towards a taxonomic revision of the genus Trollius (Ranunculaceae) in the Asian part of Russia. I. Trollius chinensis: taxonomic and geographical reconsiderations. Novosti Sist. Vyssh. Rast. 50:101-114.
  30. Song, S.J., U.S. Shin, H.J. Oh, S.Y. Kim and S.Y. Lee. 2019. Seed germination responses and interspecific variations to different incubation temperatures in eight Veronica species native to Korea. Hortic. Sci. Technol. 37(1):20-31 (in Korean). https://doi.org/10.12972/kjhst.20190003
  31. Song, Y.J., W.K. Choi and H.Y. Jin. 2015. The current condition and improvement plan of native herbaceous plants in selling online. J. People Plants Environ. 18(4):288-297 (in Korean). https://doi.org/10.11628/ksppe.2015.18.4.289
  32. Suh, S.J., J. Yu, I.B. Jang and Y.C. Kim. 2022. Effect of seed moisture content on seed storage of dehisced ginseng seeds. Korean J. Plant Res. 35(2):183-191 (in Korean).
  33. Yang, M., R.F. Wang, L.J. Liu, X. Yang, Y.S. Peng and Z.X. Sun. 2013. Contribution evaluation of the floral parts to orientin and vitexin concentrations in the flowers of Trollius chinensis. Chin. J. Nat. Med. 11(6):699-704.