The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Cardioprotection via mitochondrial transplantation supports fatty acid metabolism in ischemia-reperfusion injured rat heart

  • Jehee Jang (Department of Physiology, College of Medicine, Chung-Ang University) ;
  • Ki-Woon Kang (Divsion of Cardiology, Department of Internal Medicine, College of Medicine, Chung-Ang University Hospital) ;
  • Young-Won Kim (Department of Physiology, College of Medicine, Chung-Ang University) ;
  • Seohyun Jeong (Department of Physiology, College of Medicine, Chung-Ang University) ;
  • Jaeyoon Park (Department of Medicine, College of Medicine, Chung-Ang University) ;
  • Jihoon Park (Department of Medicine, College of Medicine, Chung-Ang University) ;
  • Jisung Moon (Department of Medicine, College of Medicine, Chung-Ang University) ;
  • Junghyun Jang (Department of Medicine, College of Medicine, Chung-Ang University) ;
  • Seohyeon Kim (Department of Medicine, College of Medicine, Chung-Ang University) ;
  • Sunghun Kim (Department of Medicine, College of Medicine, Chung-Ang University) ;
  • Sungjoo Cho (Department of Medicine, College of Medicine, Chung-Ang University) ;
  • Yurim Lee (Department of Medicine, College of Medicine, Chung-Ang University) ;
  • Hyoung Kyu Kim (Cardiovascular and Metabolic Disease Center, SMART Marine Therapeutics Center) ;
  • Jin Han (Cardiovascular and Metabolic Disease Center, SMART Marine Therapeutics Center) ;
  • Eun-A Ko (Department of Physiology, School of Medicine, Jeju National University) ;
  • Sung-Cherl Jung (Department of Physiology, School of Medicine, Jeju National University) ;
  • Jung-Ha Kim (Department of Family Medicine, College of Medicine, Chung-Ang University Hospital) ;
  • Jae-Hong Ko (Department of Physiology, College of Medicine, Chung-Ang University)
  • Received : 2024.02.21
  • Accepted : 2024.02.28
  • Published : 2024.05.01
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Abstract

In addition to cellular damage, ischemia-reperfusion (IR) injury induces substantial damage to the mitochondria and endoplasmic reticulum. In this study, we sought to determine whether impaired mitochondrial function owing to IR could be restored by transplanting mitochondria into the heart under ex vivo IR states. Additionally, we aimed to provide preliminary results to inform therapeutic options for ischemic heart disease (IHD). Healthy mitochondria isolated from autologous gluteus maximus muscle were transplanted into the hearts of Sprague-Dawley rats damaged by IR using the Langendorff system, and the heart rate and oxygen consumption capacity of the mitochondria were measured to confirm whether heart function was restored. In addition, relative expression levels were measured to identify the genes related to IR injury. Mitochondrial oxygen consumption capacity was found to be lower in the IR group than in the group that underwent mitochondrial transplantation after IR injury (p < 0.05), and the control group showed a tendency toward increased oxygen consumption capacity compared with the IR group. Among the genes related to fatty acid metabolism, Cpt1b (p < 0.05) and Fads1 (p < 0.01) showed significant expression in the following order: IR group, IR + transplantation group, and control group. These results suggest that mitochondrial transplantation protects the heart from IR damage and may be feasible as a therapeutic option for IHD.

Keywords

Acknowledgement

This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (2017R1D1A1B06035273 and 2021R1A6A3A01088465), and by the Chung-Ang University Research Scholarship Grants in 2022.

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