대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
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표준담배추출물과 Lipopolysaccharide로 유발한 만성폐쇄성폐질환 동물모델에서 쌍화탕의 폐손상 및 근감소 억제 효과

Inhibitory Effects of Ssanghwa-tang on Lung Injury and Muscle Loss in a Cigarette Smoke Extract and Lipopolysaccharide-induced Chronic Obstructive Pulmonary Disease Mouse Model

  • 최진관 (대전대학교 한의과대학 폐계내과학교실) ;
  • 양원경 (대전대학교 한의과대학 폐계내과학교실) ;
  • 이수원 (대전대학교 한의과대학 폐계내과학교실) ;
  • 우성천 (대전대학교 한의과대학 폐계내과학교실) ;
  • 김승형 (대전대학교 동서생명과학연구원) ;
  • 박양춘 (대전대학교 한의과대학 폐계내과학교실)
  • Jin-kwan Choi (Division of Respiratory Medicine, Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Won-kyung Yang (Division of Respiratory Medicine, Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Su-won Lee (Division of Respiratory Medicine, Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Seong-cheon Woo (Division of Respiratory Medicine, Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Seung-hyung Kim (Institute of Bioscience and Integrative Medicine, Daejeon University) ;
  • Yang-chun Park (Division of Respiratory Medicine, Dept. of Internal Medicine, College of Korean Medicine, Daejeon University)
  • 투고 : 2024.02.26
  • 심사 : 2024.04.02
  • 발행 : 2024.03.30
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초록

Objectives: This study evaluated the effects of Ssanghwa-tang (SHT) on lung injury and muscle loss in a COPD mouse model. Methods: C57BL/6 mice were challenged with cigarette smoke extract and lipopolysaccharide, and then treated with two concentrations of SHT (250 and 500 mg/kg). After sacrifice, the bronchoalveolar lavage fluid (BALF) or lung tissue was analyzed by cytospin, ELISA, real-time PCR, flow cytometry analysis, and H&E and Masson's trichrome staining. The grip strength of COPD mice was measured using a grip strength meter. The running time of COPD mice was measured by a treadmill test. Muscle tissue of the quadriceps was stained with H&E and Masson's trichrome staining. Results: SHT significantly inhibited the increase in neutrophil numbers in BALF and significantly decreased immune cell activity in BALF and lung tissue. It also significantly inhibited the increase in TNF-α, IL-17, and MIP2 in BALF. Real-time PCR analysis revealed that the mRNA expression of TNF-α, IL-17, MIP2, and TRPV1 in lung tissue showed a significant decrease compared with the control group. Lung tissue damage was significantly reduced in the histological analysis. The grip strength and running time of the COPD mice showed a significant decrease compared with the control group. In histological staining, SHT was found to reduce the damage to muscle tissue. Conclusions: This study indicates that SHT can be used as a therapeutic agent for COPD patients by inhibiting lung injury and muscle loss.

키워드

과제정보

이 논문은 2023학년도 대전대학교 교내학술연구비 지원에 의해 연구되었음.

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