Journal of Integrative Natural Science (통합자연과학논문집)

The Basic Science Institute Chosun University (조선대학교 기초과학연구원)
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EF-hands in CBP7 are Important in the Process of Development

  • Dahyeon Kim (Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-associated Disorder Control Technology, Chosun University) ;
  • Taeck Joong Jeon (Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-associated Disorder Control Technology, Chosun University) ;
  • Byeonggyu Park (ALBIOTEK) ;
  • Dong Yeop SHIN (INVIVO Co. Ltd.)
  • Received : 2024.02.27
  • Accepted : 2024.03.12
  • Published : 2024.03.30
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Abstract

Calcium ions play an important role in development and intracellular signaling. Dictyostelium discoideum has 14 genes encoding calcium -binding proteins (CBPs), but the function of most CBPs during development has not yet been studied. In this study, we investigated the specific functions of CBP7, one of 14 CBPs, in development using RNA interference cell lines of CBP7, cell lines overexpressing CBP7, cell lines with point mutations in the EF-hand domain, and cell lines expressing fragment proteins. was intended to reveal. CBP7 consists of 169 amino acids and contains 4EF-hand domains. The CBP7-overexpressing cells showed complete loss of developmental process. These cells remained in the single-cell growth stage under development -inducing conditions, while wild-type cells formed aggregations within 6-8h of development and eventually formed fruiting bodies. The experiments using point-mutated CBP7 protein showed that all EF-hand domains of CBP7 were important for CBP7 to function during developmental process. These results suggest that CBP7 plays an important role in developmental processes across all EF-hand domains.

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References

  1. Chisholm, R.L., and Firtel, R.A., "Insights into morphogenesis from a simple developmental system.", Nature reviews. Molecular cell biology Vol. 5, pp. 531-541, 2004. https://doi.org/10.1038/nrm1427
  2. Tanaka, Y., Nakayama, K., and Shigenobu, K., "Changes in cytosolic Ca2+ measured by use of fura-2 and contraction produced by quick stretch and various stimulants in canine cerebral artery.", Research communications in molecular pathology and pharmacology Vol. 102, pp. 79-92, 1998.
  3. Van Haastert, P.J., "Transduction of the chemotactic cAMP signal across the plasma membrane of Dictyostelium cells.", Experientia Vol. 51, pp. 1144-1154, 1995. https://doi.org/10.1007/BF01944732
  4. Chin, D., and Means, A.R., "Calmodulin: a prototypical calcium sensor.", Trends in cell biology Vol. 10, pp. 322-328, 2000. https://doi.org/10.1016/S0962-8924(00)01800-6
  5. Sakamoto, H., Nishio, K., Tomisako, M., Kuwayama, H., Tanaka, Y., Suetake, I., Tajima, S., Ogihara, S., Coukell, B., and Maeda, M., "Identification and characterization of novel calcium-binding proteins of Dictyostelium and their spatial expression patterns during development.", Development, growth & differentiation Vol. 45, pp. 507-514, 2003. https://doi.org/10.1111/j.1440-169X.2003.00718.x
  6. Gifford, J.L., Walsh, M.P., and Vogel, H.J., "Structures and metal-ion-binding properties of the Ca2+-binding helix-loop-helix EF-hand motifs.", The Biochemical journal Vol. 405, pp. 199-221, 2007. https://doi.org/10.1042/BJ20070255
  7. Mishig-Ochiriin, T., Lee, C.H., Jeong, S.Y., Kim, B.J., Choi, C.H., Yim, H.S., and Kang, S.O., "Calcium-induced conformational changes of the recombinant CBP3 protein from Dictyostelium discoideum.", Biochimica et biophysica acta Vol. 1748, pp. 157-164, 2005. https://doi.org/10.1016/j.bbapap.2004.12.018
  8. Dharamsi, A., Tessarolo, D., Coukell, B., and Pun, J., "CBP1 associates with the Dictyostelium cytoskeleton and is important for normal cell aggregation under certain developmental conditions.", Experimental cell research Vol. 258, pp. 298-309, 2000. https://doi.org/10.1006/excr.2000.4950
  9. Lee, C.H., Jeong, S.Y., Kim, B.J., Choi, C.H., Kim, J.S., Koo, B.M., Seok, Y.J., Yim, H.S., and Kang, S.O., "Dictyostelium CBP3 associates with actin cytoskeleton and is related to slug migration.", Biochimica et biophysica acta Vol. 1743, pp. 281-290, 2005. https://doi.org/10.1016/j.bbamcr.2005.01.003
  10. Myre, M.A., and O'Day, D.H., "Dictyostelium calcium-binding protein 4a interacts with nucleomorphin, a BRCT-domain protein that regulates nuclear number.", Biochemical and biophysical research communications Vol. 322, pp. 665-671, 2004. https://doi.org/10.1016/j.bbrc.2004.07.168
  11. Itoh, M., Noguchi, M., and Maeda, Y., "Overexpression of CAF1 encoding a novel Ca2+-binding protein stimulates the transition of Dictyosteliumcells from growth to differentiation.", Development, growth & differentiation Vol. 40, pp. 677-683, 1998.