Journal of Veterinary Science

  • 제25권2호
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  • Pages.21.1-21.15
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  • 2024
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Peste des petits ruminants virus infection induces endoplasmic reticulum stress and apoptosis via IRE1-XBP1 and IRE1-JNK signaling pathways

  • Shuyi Yuan (College of Coastal Agricultural Science of Guangdong Ocean University) ;
  • Yanfen Liu (College of Coastal Agricultural Science of Guangdong Ocean University) ;
  • Yun Mu (College of Coastal Agricultural Science of Guangdong Ocean University) ;
  • Yongshen Kuang (College of Coastal Agricultural Science of Guangdong Ocean University) ;
  • Shaohong Chen (College of Food Science and Technology of Guangdong Ocean University) ;
  • Yun-Tao Zhao (College of Food Science and Technology of Guangdong Ocean University) ;
  • You Liu (College of Food Science and Technology of Guangdong Ocean University)
  • 투고 : 2023.09.28
  • 심사 : 2024.01.10
  • 발행 : 2024.03.31
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초록

Background: Peste des petits ruminants (PPR) is a contagious and fatal disease of sheep and goats. PPR virus (PPRV) infection induces endoplasmic reticulum (ER) stress-mediated unfolded protein response (UPR). The activation of UPR signaling pathways and their impact on apoptosis and virus replication remains controversial. Objectives: To investigate the role of PPRV-induced ER stress and the IRE1-XBP1 and IRE1-JNK pathways and their impact on apoptosis and virus replication. Methods: The cell viability and virus replication were assessed by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, immunofluorescence assay, and Western blot. The expression of ER stress biomarker GRP78, IRE1, and its downstream molecules, PPRV-N protein, and apoptosis-related proteins was detected by Western blot and quantitative reverse transcription-polymerase chain reaction, respectively. 4-Phenylbutyric acid (4-PBA) and STF-083010 were respectively used to inhibit ER stress and IRE1 signaling pathway. Results: The expression of GRP78, IRE1α, p-IRE1α, XBP1s, JNK, p-JNK, caspase-3, caspase-9, Bax and PPRV-N were significantly up-regulated in PPRV-infected cells, the expression of Bcl-2 was significantly down-regulated. Due to 4-PBA treatment, the expression of GRP78, p-IRE1α, XBP1s, p-JNK, caspase-3, caspase-9, Bax, and PPRV-N were significantly downregulated, the expression of Bcl-2 was significantly up-regulated. Moreover, in PPRV-infected cells, the expression of p-IRE1α, p-JNK, Bax, and PPRV-N was significantly decreased, and the expression of Bcl-2 was increased in the presence of STF-083010. Conclusions: PPRV infection induces ER stress and IRE1 activation, resulting in apoptosis and enhancement of virus replication through IRE1-XBP1s and IRE1-JNK pathways.

키워드

과제정보

The authors thank Hualong Deng, Xiaoyou Jiang, Puhong Ying, and Tiantian Sun, for their help in the execution of the project.

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