식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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First Report of Pectobacterium aroidearum Causing Soft Rot on Ficus carica in Korea

  • Kyoung-Taek Park (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Leonid N. Ten (Institute of Plant Medicine, Kyungpook National University) ;
  • Soo-Min Hong (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Song-Woon Nam (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Chang-Gi Back (Department of Environmental Horticulture and Landscape Architecture, Environmental Horticulture, Dankook University) ;
  • Seung-Yeol Lee (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Hee-Young Jung (College of Agriculture and Life Sciences, Kyungpook National University)
  • 투고 : 2023.11.29
  • 심사 : 2024.01.15
  • 발행 : 2024.03.31
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초록

In July 2021, symptoms of soft rot were observed on the stems of Ficus carica in Yeongam, Jeollanamdo, Korea. To accurately diagnose the cause, infected stem was collected and bacterial strain was isolated. Among these, the pathogenic strain KNUB-08-21 was identified as Pectobacterium aroidearum through 16S rRNA gene sequencing and phylogenetic analysis based on the concatenated sequences of the dnaX, leuS, and recA genes. The affiliation of the isolate with this bacterial species was also confirmed by its biochemical characteristics obtained using API ID 32 GN system. Artificial inoculation confirmed the strain's pathogenicity in figs, causing significant damage to both stems and fruits. To our knowledge, this is the first report of P. aroidearum causing soft rot disease in F. carica in Korea.

키워드

과제정보

This work was carried out with the support of "Cooperative Research Program for Agriculture Science and Technology Development (Project No. RS-2021-RD010123)" Rural Development Administration, Republic of Korea.

참고문헌

  1. Adeolu, M., Alnajar, S., Naushad, S. and Gupta, R. S. 2016. Genome-based phylogeny and taxonomy of the 'Enterobacteriales': proposal for Enterobacterales ord. nov. divided into the families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov. Int. J. Syst. Evol. Microbiol. 66: 5575-5599. https://doi.org/10.1099/ijsem.0.001485
  2. Badgujar, S. B., Patel, V. V., Bandivdekar, A. H. and Mahajan, R. T. 2014. Traditional uses, phytochemistry and pharmacology of Ficus carica: a review. Pharm. Biol. 52: 1487-1503.
  3. Bouzar, H. and Jones, J. B. 2001. Agrobacterium larrymoorei sp. nov., a pathogen isolated from aerial tumours of Ficus benjamina. Int. J. Syst. Evol. Microbiol. 51: 1023-1026.
  4. Campoverde, E. V. and Palmateer, A. J. 2011. A severe outbreak of Xanthomonas on Ficus elastica in South Florida. Proc. Fla. State Hort. Soc. 124: 321-322.
  5. Charkowski, A. O. 2018. The changing face of bacterial soft-rot diseases. Annu. Rev. Phytopathol. 56: 269-288. https://doi.org/10.1146/annurev-phyto-080417-045906
  6. Chen, L. R., Lin, P. R. and Huang, C. J. 2020. First report of Pectobacterium aroidearum causing soft rot disease of white calla lily in Taiwan. Plant Dis. 104: 563.
  7. Elboutahiri, N., Thami-Alami, I., Zaid, E. and Udupa, S. M. 2009. Genotypic characterization of indigenous Sinorhizobium meliloti and Rhizobium sullae by rep-PCR, RAPD and ARDRA analyses. Afr. J. Biotechnol. 8: 979-985.
  8. Kumar, S., Stecher, G. and Tamura, K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33: 1870-1874. https://doi.org/10.1093/molbev/msw054
  9. Li, L., Yuan, L., Zhao, Y., Shi, Y., Chai, A., Xie, X. et al. 2022. Emergence of bacterial soft rot in calla lily caused by Pectobacterium aroidearum in China. Crop Prot. 152: 105854.
  10. Ma, B., Hibbing, M. E., Kim, H. S., Reedy, R. M., Yedidia, I., Breuer, J. et al. 2007. The host range and molecular phylogenies of the soft rot enterobacterial genera Pectobacterium and Dickeya. Phytopathology 97: 1150-1163. https://doi.org/10.1094/PHYTO-97-9-1150
  11. Mikicinski, A., Warabieda, M., Nowak, J. S. and Pulawska, J. 2023. First report on Pectobacterium aroidearum, a new pathogen causing soft rot on alocasia (Alocasia amazonica) in Poland. J. Plant Pathol. 105: 1169-1170.
  12. Moraes, A. J. G., Souza, E. B., Mariano, R. L. R., Silva, A. M. F., Lima, N. B., Peixoto, A. R. et al. 2017. First report of Pectobacterium aroidearum and Pectobacterium carotovorum subsp. brasiliensis causing soft rot of Cucurbita pepo in Brazil. Plant Dis. 101: 379-380. https://doi.org/10.1094/PDIS-08-16-1168-PDN
  13. Mousavi, S. A., Hasanzadeh, N. and Abdollahi, P. 2020. Identification and characterization of bacterial agents causing moderate damage and souring of the fig fruits. J. Plant Pathol. Microbiol. 11: 514.
  14. Nabhan, S., de Boer, S. H., Maiss, E. and Wydra, K. 2013. Pectobacterium aroidearum sp. nov., a soft rot pathogen with preference for monocotyledonous plants. Int. J. Syst. Evol. Microbiol. 63: 2520-2525.
  15. Portier, P., Pedron, J., Taghouti, G., Dutrieux, C. and Barny, M. A. 2020. Updated taxonomy of Pectobacterium genus in the CIRM-CFBP bacterial collection: when newly described species reveal "old" endemic population. Microorganisms 8: 1441.
  16. Portier, P., Pedron, J., Taghouti, G., Fischer-Le Saux, M., Caullireau, E., Bertrand, C. et al. 2019. Elevation of Pectobacterium carotovorum subsp. odoriferum to species level as Pectobacterium odoriferum sp. nov., proposal of Pectobacterium brasiliense sp. nov. and Pectobacterium actinidiae sp. nov., emended description of Pectobacterium carotovorum and description of Pectobacterium versatile sp. nov., isolated from streams and symptoms on diverse plants. Int.J. Syst. Evol. Microbiol. 69: 3207-3216. https://doi.org/10.1099/ijsem.0.003611
  17. Singh, D., Singh, B. and Goel, R. K. 2011. Traditional uses, phytochemistry and pharmacology of Ficus religiosa: a review. J. Ethnopharmacol. 134: 565-583.
  18. Slawiak, M., van Beckhoven, J. R. C. M., Speksnijder, A. G. C. L., Czajkowski, R., Grabe, G. and van der Wolf, J. M. 2009. Biochemical and genetical analysis reveal a new clade of biovar 3 Dickeya spp. strains isolated from potato in Europe. Eur. J. Plant Pathol. 125: 245-261. https://doi.org/10.1007/s10658-009-9479-2
  19. Statistics Korea. 2023. Korean Statistical Information Service. URL https://kostat.go.kr/unifSearch/search.es [3 August 2023].
  20. Sun, M., Liu, H., Huang, J., Peng, J., Fei, F., Zhang, Y. et al. 2019. A loop-mediated isothermal amplification assay for rapid detection of Pectobacterium aroidearum that causes soft rot in Konjac. Int. J. Mol. Sci. 20: 1937.
  21. Waleron, M., Waleron, K., Podhajska, A. J. and Lojkowska, E. 2002. Genotyping of bacteria belonging to the former Erwinia genus by PCR-RFLP analysis of a recA gene fragment. Microbiology 148: 583-595. https://doi.org/10.1099/00221287-148-2-583
  22. Wei, H. Y., Yang, M., Pei, W. H., Wei, W., Huang, F. Y., Liu, J. N. et al. 2020. First report of Pectobacterium aroidearum causing soft rot of Amorphophallus konjac in China. Plant Dis. 104: 969.
  23. Weisburg, W. G., Barns, S. M., Pelletier, D. A. and Lane, D. J. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173: 697-703. https://doi.org/10.1128/jb.173.2.697-703.1991
  24. Xie, H., Li, X. Y., Ma, Y. L. and Tian, Y. 2018. First report of Pectobacterium aroidearum causing soft rot of Chinese cabbage in China. Plant Dis. 102: 674.
  25. Xu, P. D., Wei, D. D., Li, Z. P., Qin, C. X., Li, X., Lin, C. H. et al. 2020. First report of bacterial soft rot on Syngonium podophyllum caused by Pectobacterium aroidearum in China. Plant Dis. 104: 2720-2721. https://doi.org/10.1094/PDIS-03-20-0552-PDN