식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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목이버섯 배지 오염 곰팡이균의 분리, 동정 및 생물학적 방제제 선발

Isolation and Identification of Competitive Fungi on Medium for Black Wood Ear Mushroom in Korea and In Vitro Selection of Potential Biocontrol Agents

  • 김서연 (순천대학교 생명산업과학대학 식물의학과) ;
  • 조미주 (순천대학교 생명산업과학대학 식물의학과) ;
  • 안선민 (순천대학교 생명산업과학대학 식물의학과) ;
  • 박지윤 (순천대학교 생명산업과학대학 식물의학과) ;
  • 박지원 (순천대학교 생명산업과학대학 식물의학과) ;
  • 홍성국 (순천대학교 생명산업과학대학 식물의학과) ;
  • 김지우 (순천대학교 생명산업과학대학 식물의학과) ;
  • 차주훈 (삼광버섯영농조합) ;
  • 노유진 (순천대학교 생명산업과학대학 식물의학과) ;
  • 김다솜 (국립생물자원관) ;
  • 전미진 (국립생물자원관) ;
  • 지원재 (국립생물자원관) ;
  • 박숙영 (순천대학교 생명산업과학대학 식물의학과)
  • Seoyeon Kim (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University) ;
  • Miju Jo (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University) ;
  • Sunmin An (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University) ;
  • Jiyoon Park (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University) ;
  • Jiwon Park (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University) ;
  • Sungkook Hong (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University) ;
  • Jiwoo Kim (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University) ;
  • Juhoon Cha (Samkwang Mushroom Farmer's Association) ;
  • Yujin Roh (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University) ;
  • Da Som Kim (Species Diversity Research Division, Biodiversity Research Department, National Institute of Biological Resources) ;
  • Mi jin Jeon (Species Diversity Research Division, Biodiversity Research Department, National Institute of Biological Resources) ;
  • Won-Jae Chi (Species Diversity Research Division, Biodiversity Research Department, National Institute of Biological Resources) ;
  • Sook-Young Park (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University)
  • 투고 : 2023.11.15
  • 심사 : 2024.02.07
  • 발행 : 2024.03.31
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초록

목이버섯(A. auricula-judae)은 중국, 일본, 한국에서 경제적으로 중요한 버섯이다. 인공 배지에서 목이버섯을 재배할 경우 자연적으로 나무에서 키울 때보다 시간과 비용 면에서 더 효율적이다. 그러나 배지 재배의 경우 빠르게 자라는 곰팡이에 감염할 경우 성장이 느린 목이버섯이 배지 경쟁에서 밀려나 경제적 손실이 발생한다. 이 연구에서는 전라남도 장흥과 순천에서 목이버섯 재배용 배지에 감염한 푸른곰팡이들을 분리 및 동정하였다. 총 54개의 균주를 수집한 뒤 ITS 염기서열 분석 및 형태학적 동정을 수행하였다. 총 54개 균주 중 Trichoderma spp.가 92.6%, Penicillium spp.가 5.6%, Talaromyces sp.가 1.8%였다. 친환경 방제제를 선발하기 위해 우리는 6개의 Streptomyces spp. 균주를 선발하고 Benomyl을 대조군으로 사용하여 분리균주인 Trichoderma spp.와 Penicillium spp.에 대한 길항성 검정을 수행하였다. 이 중 Streptomyces sp. 203-3이 Trichoderma spp.와 Penicillium spp.에 가장 높은 균사저지 효과를 보여, 목이버섯 배지에 감염하는 곰팡이에 대한 잠재적인 방제제로의 가능성을 보였다.

Black wood ear mushroom (Auricularia auricula-judae) is one of the most economically important mushrooms in China, Japan, and Korea. The cultivation of wood ear mushrooms on artificial substrates is more efficient in terms of time and cost compared with their natural growth on trees. However, if the substrate cultivation is infected by fast-growing fungi, the relatively slow-growing ear mushroom will be outcompeted, leading to economic losses. In this study, we investigated the competitive fungal isolates from substrates infected with fast-growing fungi for the cultivation of ear mushrooms in Jangheung and Sunchon, Korea. We collected 54 isolates and identified them by sequencing their internal transcribed spacer region with morphological identification. Among the isolates, the dominant isolates were Trichoderma spp. (92.6%), Penicillium spp. (5.6%), and Talaromyces sp. (1.8%). To find an appropriate eco-friendly biocontrol agent, we used five Streptomyces spp. and Benomyl, as controls against Trichoderma spp. and Penicillium spp. Among the six Streptomyces spp., Streptomyces sp. JC203-3 effectively controlled the fungi Trichoderma spp. and Penicillium spp., which pose a significant problem for the substrates of black wood ear mushrooms. This result indicated that this Streptomyces sp. JC203-3 can be used as biocontrol agents to protect against Trichoderma and Penicillium spp.

키워드

과제정보

This paper was supported by National Institute of Biological Resources (grant number: NIBR202304106).

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