Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Paprika Damping-off Outbreak Caused by Fusarium oxysporum Contaminated Seeds in Cheorwon Province in 2023

2023 파프리카 종자의 Fusarium oxysporum 오염에 의한 철원지역 파프리카 모잘록병 대발생

  • Miah Bae (Division of Region-Specific Industries Fostering, Cheorwon Plasma Research Institute) ;
  • Namsuk Kim (Division of Agricultural Policy, Cheorwon Agricultural Technology Center) ;
  • Sang Woo Kim (Division of Region-Specific Industries Fostering, Cheorwon Plasma Research Institute) ;
  • Sangyeon Ju (Division of Region-Specific Industries Fostering, Cheorwon Plasma Research Institute) ;
  • Byungyeon Kim (Division of Region-Specific Industries Fostering, Cheorwon Plasma Research Institute) ;
  • Soo Man Hwang (Division of Agricultural Policy, Cheorwon Agricultural Technology Center) ;
  • MeeKyoung Kim (Division of Agricultural Policy, Cheorwon Agricultural Technology Center) ;
  • Mi-Ri Park (Division of Region-Specific Industries Fostering, Cheorwon Plasma Research Institute)
  • 배미아 ((재)철원플라즈마산업기술연구원) ;
  • 김남숙 (철원군농업기술센터) ;
  • 김상우 ((재)철원플라즈마산업기술연구원) ;
  • 주상연 ((재)철원플라즈마산업기술연구원) ;
  • 김병연 ((재)철원플라즈마산업기술연구원) ;
  • 황수만 (철원군농업기술센터) ;
  • 김미경 (철원군농업기술센터) ;
  • 박미리 ((재)철원플라즈마산업기술연구원)
  • Received : 2024.02.06
  • Accepted : 2024.02.16
  • Published : 2024.03.31
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Abstract

In 2023, symptoms like damping-off disease were observed in 74 paprika growing in greenhouses in Cheorwon-gun, Gangwon-do, Korea. In this study, we tried to find the cause of the damping-off disease outbreak. We collected symptomatic seedlings and observed the typical crescent-shaped conidia of Fusarium oxysporum by microscope. To confirm the presence of F. oxysporum in the samples, polymerase chain reaction was performed using primers specific for F. oxysporum; the resulting sequence showed 99.11% identity with F. oxysporum. To confirm the pathogenicity of the F. oxysporum (CW) isolated from the samples, healthy paprika plants were inoculated with F. oxysporum CW and damping-off symptoms were observed 2 weeks later. To investigate whether the damping-off disease outbreak in Cheorwon-gun was caused by F. oxysporum-contaminated seeds, 100 paprika seeds were disinfected and placed in Murashige and Skoog medium. Typical pink F. oxysporum hyphae were found only in control non-disinfected seeds. An 18S rRNA-based and a TEF genebased phylogenetic analysis showed that the F. oxysporum CW isolate was not grouped with a F. oxysporum isolate reported from Cheorwon-gun in 2019. This study is the first report that an outbreak of damping-off disease in paprika in Cheorwon-gun, Gangwon-do, Korea, was caused by contamination of F. oxysporum seeds.

2023년 강원특별자치도 철원군의 파프리카 농가 74곳에서 특정 품종의 유묘에 줄기가 잘록해져서 쓰러지는 모잘록병 증상을 보이는 병이 대발생함에 따라 본 연구에서는 모잘록병의 원인을 규명하고자 하였다. 모잘록병으로 의심되는 유묘에서 현미경으로 관찰 시, 초승달 모양의 F. oxysporum의 전형적인 분생포자가 관찰되었다. 이에 따라 F. oxysporum 특이 프라이머를 이용하여 PCR을 수행하여 양성 밴드를 확보하였으며, 염기서열을 분석한 결과 F. oxysporum의 유전자임이 확인되었다. 농가에서 분리한 F. oxysporum의 병원성의 재현성을 확인하기 위해 농가에서 분리한 F. oxysporum 균주를 다른 파프리카 품종의 건전주에 접종하여 모잘록병이 발생함을 확인하였다. 이번 모잘록병의 대발생이 오염된 종자에 의한 것인지, 농가 환경에서 감염되어 확산된 것인지의 여부를 확인하기 위해, 표면살균 처리한 파프리카 종자와 살균하지 않은 무처리 종자를 MS 배지에 각각 치상하여 배양하였다. 그 결과, 무처리 종자배지에서만 분홍색의 전형적인 F. oxysporum의 균사가 확인되었다. 또한, 농가에서 분리한 균주, 농가에서 분리한 균주를 접종하여 얻은 균주 그리고 같은 파프리리카 품종의 제조생산번호가 같은 미개봉 종자에서 분리한 균주가 동일한 F. oxysporum인지를 확인하기 위해 18S rRNA region의 염기서열을 이용하여 분자계통분류학적 분석을 진행한 결과, 결과 3종의 F. oxysporum 균주가 같은 그룹으로 분류되었으며, 기존에 철원군에서 보고된 F. oxysporum과는 다른 그룹임을 확인하였다. 이는 파프리카의 모잘록병 대발생의 원인은 F. oxysporum에 오염된 종자에 의한 것임을 증명하였다.

Keywords

Acknowledgement

This research was supported in part by grants from Research Program funded by Cheorwon Agricultural Technology Center.

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