Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Purification and Antibacterial Activity of Compound Derived from Marine Actinomycetes

해양 방선균 유래 물질의 정제 및 항균 활성

  • 정성윤 (대구가톨릭대학교 바이오메디컬학과)
  • Received : 2024.02.02
  • Accepted : 2024.03.12
  • Published : 2024.03.31
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Abstract

Antibiotics are substances produced by microorganisms that kill or inhibit and are essential for infectious diseases management. This study aimed to provide basic data for overcoming antibiotic resistance in the marine bacterium LJ-18. The API 20NE and API 50CH kits were used to identify this microorganism. Morphological, physiological, and biochemical properties were investigated using MacFaddin's manuals. Subsequently, isolated LJ-18 was found to belong to a genus of Streptomyces that forms mycelia. LJ-18 also grew well at 28-32℃ on modified Bennett's agar. To isolate and purify the antibacterial compound, LJ-18 culture was divided into ethyl acetate and distilled water fractions. Considerable antimicrobial activity against various pathogenic microorganisms, including methicillin-resistant Staphylococcus aureus (MRSA), was confirmed in the C18 ODS open column fractions. Peak 2 compound was obtained using reversed-phase HPLC. As a result, this compound had a significant antimicrobial activity against various pathogenic microorganisms. In particular, it showed strong activity against MRSA, Mycobacterium smegmatis, Bacillus subtilis, Bacillus cereus, and Staphylococcus aureus.

Keywords

Acknowledgement

이 논문은 2021년도 대구가톨릭대학교 학술연구비 지원에 의한 것이며 이에 감사드립니다(20211191).

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