Korean Journal of Acupuncture

  • 제41권1호
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  • Pages.7-15
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  • 2024
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  • 2287-3368(pISSN)
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  • 2287-3376(eISSN)
경락경혈학회 (Society for Meridian and Acupoint)
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형개 추출물의 시스템 약리학적 분석과 비소세포폐암세포에 대한 증식 억제효과

Systems Pharmacological Approach to Identification of Schizonepeta teunifolia Extract via Active Ingredients Analysis and Cytotoxicity Effect on A549 Cell Lines

  • 양가람 (부산대학교 한의학전문대학원 약물의학부) ;
  • 추지은 ((주)비체담 기업부설연구소) ;
  • 김윤숙 (부산대학교 장수웰빙연구소) ;
  • 안원근 (부산대학교 한의학전문대학원 약물의학부)
  • Ga Ram Yang (Department of Pharmacology, College of Korean Medicine, Pusan National University) ;
  • Ji Eun Choo (Research and Development Institute, Bichedam Co., Ltd.) ;
  • Youn Sook Kim (Research Institute for Longevity and Well-Being, Pusan National University) ;
  • Won Gun Ahn (Department of Pharmacology, College of Korean Medicine, Pusan National University)
  • 투고 : 2024.02.16
  • 심사 : 2024.03.17
  • 발행 : 2024.03.27
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초록

Objectives : This study aimed to predict the effectiveness and potential of Schizonepeta tenuifolia as an anticancer treatment for non-small cell lung cancer through network-based pharmacology and cellular experiment. Methods : To identify the major bioactive compounds in Schizonepeta tenuifolia, we used the Traditional Chinese Medicine Systems. The target genes for the cancer treatment were selected using the UniProt database and the networked using Cytoscape. We performed functional enrichment analysis based on the Gene Ontology Biological Process and Kyoto Encyclopedia of Genes and Genomes Pathways to predict the mechanisms. To investigate the effect of Schizonepeta tenuifolia on lung cancer cell growth, we treated A549 cells, a lung cancer cell line, with different concentrations of the drug and used the MTT assay for cell viability. Results : Research has shown that the most effective mechanism of active compounds from Schizonepeta tenuifolia is through the pathway of cancer. The results of the network pharmacology analysis indicate that Schizonepeta tenuifolia has potential medicinal value as an adjuvant in anticancer treatment. The concentration-dependent inhibition of cell viability was observed on A549 cells. Furthermore, synergistic anticancer activity with Doxorubicin was also observed. Conclusions : Through a network pharmacological approach, Schizonepeta tenuifolia was predicted to have potential as an anticancer agent, and its efficacy was experimentally demonstrated using A549 cells. These findings suggest that Schizonepeta tenuifolia is a promising candidate for future research.

키워드

과제정보

This research was supported by (Basic Science Research Program) through the (National Research Foundation of Korea [NRF]) funded by the (Ministry of Education) under grant (NRF-2021R1I1A 1A01058697).

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