Parasites, Hosts and Diseases

  • 제62권1호
  • /
  • Pages.117-130
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  • 2024
  • /
  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Diversity of midgut microbiota in ticks collected from white-tailed deer (Odocoileus virginianus) from northern Mexico

  • Zinnia Judith Molina-Garza (Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, Laboratorio de Patologia Molecular y Experimental, Ciudad Universitaria) ;
  • Mariana Cuesy-Leon (Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, Laboratorio de Patologia Molecular y Experimental, Ciudad Universitaria) ;
  • Lidia Baylon-Pacheco (Departamento de Infectomica y Patogenesis Molecular, Centro de Investigacion y de Estudios Avanzados de IPN) ;
  • Jose Luis Rosales-Encina (Departamento de Infectomica y Patogenesis Molecular, Centro de Investigacion y de Estudios Avanzados de IPN) ;
  • Lucio Galaviz-Silva (Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, Laboratorio de Patologia Molecular y Experimental, Ciudad Universitaria)
  • 투고 : 2023.01.17
  • 심사 : 2023.11.08
  • 발행 : 2024.02.29
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초록

Ticks host different pathogens as endosymbiont and nonpathogenic microorganisms and play an important role in reproductive fitness and nutrient provision. However, the bacterial microbiomes of white-tailed deer ticks have received minimal attention. This study aimed to examine the bacterial microbiome of ticks collected from Odocoileus virginianus on the Mexico-United States border to assess differences in microbiome diversity in ticks of different species, sexes, and localities. Five different tick species were collected: Rhipicephalus microplus, Dermacentor nitens, Otobius megnini, Amblyomma cajennense, and A. maculatum. The tick microbiomes were analyzed using next-generation sequencing. Among all tick species, the most predominant phylum was Proteobacteria, followed by Actinobacteria and Firmicutes. The ticks from Tamaulipas and Nuevo León presented the highest bacterial species diversity. Acinetobacter johnsonii and A. lwoffii were the common bacterial species in the microbiome of all ticks, Coxiella were present in R. microplus, and Dermacentor nitens also exhibited a Francisella-like endosymbiont. The microbiome of most females in D. nitens was less diverse than that of males, whereas R. microplus occurs in females, suggesting that microbiome diversity is influenced by sex. In the bacterial communities of A. maculatum and O. megnini, Candidatus Midichloria massiliensis, and Candidatus Endoecteinascidia fumentensis were the most predominant endosymbionts. These results constitute the initial report on these bacteria, and this is also the first study to characterize the microbiome of O. megnini.

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과제정보

We thank the staff from Organizacion de Vida Silvestre S.A. de C.V. (OVIS) for their participation during tick collection. This research was supported by FOINS PN-CONACyT (grant #3157) and PAICyT-UANL. We are also grateful to CONACyT for the scholarship granted to the second author (MCL) during her Ph.D. degree. Raw sequence data are available in NCBI GenBank under Bioproject PRJNA88132.

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