Parasites, Hosts and Diseases

  • 제62권1호
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  • Pages.85-97
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  • 2024
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Morphological and molecular characteristics of Paralecithodendrium longiforme (Digenea: Lecithodendriidae) adults and cercariae from Chinese pipistrelle bats and viviparid snails in Thailand

  • Thitichai Arttra (Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University) ;
  • Pheravut Wongsawad (Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University) ;
  • Chalobol Wongsawad (Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University) ;
  • Nattawadee Nantarat (Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University) ;
  • Preeyaporn Butboonchoo (Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University) ;
  • Jong-Yil Chai (Department of Tropical Medicine and Parasitology, Seoul National University College of Medicine)
  • 투고 : 2023.10.27
  • 심사 : 2023.12.28
  • 발행 : 2024.02.29
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초록

This study aimed to describe the morphological and molecular characteristics of Paralecithodendrium longiforme (Digenea: Lecithodendriidae) adults and cercariae isolated in Thailand. Adult flukes were isolated from the Chinese pipistrelle bat (Hypsugo sp.), and cercariae were detected in the viviparid snail (Filopaludina martensi martensi) from Chiang Mai province. The morphological characteristics were observed and described using conventional methods, and the molecular characteristics with internal transcribed spacer 2 (ITS2) and 28S rDNA gene sequences. The adult flukes were fusiform, 0.84-0.98 mm in length, and 0.37-0.49 mm in width, and were distinguishable from other species by the presence of longitudinal uterine coils. The cercariae were nonvirgulate xiphidiocercariae, with the oral sucker bigger than the acetabulum, the tail without fin fold, a body size of 117.5-138.3×48.3-52.2 ㎛, and a tail size of 100.7-103.7×15.0-18.9 ㎛. Molecular studies revealed that the adults and cercariae shared 99.3% (ITS2) and 99.6% (28S rDNA) homology with each other. They were phylogenetically close to P. longiforme with an identity of 94.5% for ITS2 and 98.7% for 28S rDNA. This study provides new information on the natural definitive host and first intermediate host of P. longiforme in Thailand. The discovery of its cercarial stage in Filopaludina snails highlights the importance of monitoring the associated second intermediate host and prevention and control of this potentially zoonotic trematode.

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과제정보

This research was supported by Chiang Mai University. The authors acknowledge the Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University for their facilities and laboratory processing.

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