Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Integrated analysis of transcriptome and milk metagenome in subclinical mastitic and healthy cows

  • Jinning Zhang (Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University) ;
  • Xueqin Liu (Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University) ;
  • Tahir Usman (College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University) ;
  • Yongjie Tang (Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University) ;
  • Siyuan Mi (Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University) ;
  • Wenlong Li (Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University) ;
  • Mengyou Yang (Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University) ;
  • Ying Yu (Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University)
  • Received : 2021.11.01
  • Accepted : 2022.01.18
  • Published : 2024.04.01
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Abstract

Objective: Abnormally increased somatic cell counts (SCCs) in milk is usually a sign of bovine subclinical mastitis. Mutual interaction between the host and its associated microbiota plays an important role in developing such diseases. The main objective of this study was to explore the difference between cows with elevated SCCs and healthy cattle from the perspective of host-microbe interplay. Methods: A total of 31 milk samples and 23 bovine peripheral blood samples were collected from Holstein dairy cattle to conduct an integrated analysis of transcriptomic and metagenomics. Results: The results showed that Ralstonia and Sphingomonas were enriched in cows with subclinical mastitis. The relative abundance of the two bacteria was positively correlated with the expression level of bovine transcobalamin 1 and uridine phosphorylase 1 encoding gene. Moreover, functional analysis revealed a distinct alternation in some important microbial biological processes. Conclusion: These results reveal the relative abundance of Ralstonia and Sphingomonas other than common mastitis-causing pathogens varied from healthy cows to those with subclinical mastitis and might be associated with elevated SCCs. Potential association was observed between bovine milk microbiota composition and the transcriptional pattern of some genes, thus providing new insights to understand homeostasis of bovine udder.

Keywords

Acknowledgement

The authors wish to thank all the members of Molecular and Quantitative Genetics Laboratory (China Agricultural University) that may contribute to this study and Novogene Technology (Beijing, China) for the sequencing services. The financial support provided by National Natural Science Foundation of China-Pakistan Science Foundation (NSFCPSF) Joint Project (31961143009) National Key R&D Program of China (2021YFD1200903 and Beijing Natural Science Foundation (6182021) is acknowledged.

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