Animal Bioscience

  • 제37권4호
  • /
  • Pages.655-667
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  • 2024
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Rumen fermentation and microbial diversity of sheep fed a high-concentrate diet supplemented with hydroethanolic extract of walnut green husks

  • Huan Wei (Laboratory of Nutrition for Meat & Dairy Herbivore, College of Animal Science, Xinjiang Agricultural University) ;
  • Jiancheng Liu (Laboratory of Nutrition for Meat & Dairy Herbivore, College of Animal Science, Xinjiang Agricultural University) ;
  • Mengjian Liu (Laboratory of Nutrition for Meat & Dairy Herbivore, College of Animal Science, Xinjiang Agricultural University) ;
  • Huiling Zhang (Laboratory of Nutrition for Meat & Dairy Herbivore, College of Animal Science, Xinjiang Agricultural University) ;
  • Yong Chen (Laboratory of Nutrition for Meat & Dairy Herbivore, College of Animal Science, Xinjiang Agricultural University)
  • 투고 : 2023.06.10
  • 심사 : 2023.10.20
  • 발행 : 2024.04.01
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초록

Objective: This study aimed to assess the impact of a hydroethanolic extract of walnut green husks (WGH) on rumen fermentation and the diversity of bacteria, methanogenic archaea, and fungi in sheep fed a high-concentrate diet. Methods: Five healthy small-tailed Han ewes with permanent rumen fistula were selected and housed in individual pens. This study adopted a self-controlled and crossover design with a control period and an experimental period. During the control period, the animals were fed a basal diet (with a ratio of concentrate to roughage of 65:35), while during the treatment period, the animals were fed the basal diet supplemented with 0.5% hydroethanolic extract of WGH. Fermentation parameters, digestive enzyme activities, and microbial diversity in rumen fluid were analyzed. Results: Supplementation of hydroethanolic extract of WGH had no significant effect on feed intake, concentrations of total volatile fatty acids, isovalerate, ammonia nitrogen, and microbial protein (p>0.05). However, the ruminal pH, concentrations of acetate, butyrate and isobutyrate, the ratio of acetate to propionate, protozoa count, and the activities of filter paper cellulase and cellobiase were significantly increased (p<0.05), while concentrations of propionate and valerate were significantly decreased (p<0.05). Moreover, 16S rRNA gene sequencing revealed that the relative abundance of rumen bacteria Christensenellaceae R7 group, Saccharofermentans, and Ruminococcaceae NK4A214 group were significantly increased, while Ruminococcus gauvreauii group, Prevotella 7 were significantly decreased (p<0.05). The relative abundance of the fungus Pseudomonas significantly increased, while Basidiomycota, Fusarium, and Alternaria significantly decreased (p<0.05). However, there was no significant change in the community structure of methanogenic archaea. Conclusion: Supplementation of hydroethanolic extract of WGH to a high-concentrate diet improved the ruminal fermentation, altered the structure of ruminal bacterial and fungal communities, and exhibited beneficial effects in alleviating subacute rumen acidosis of sheep.

키워드

과제정보

This research was supported by the Open Project of Key Laboratory of Xinjiang (2020D04005) and the Special Project of the Central Government Guidance on Local Science and Technology Development (ZYYD2023B09).

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