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Gene expression profiles of skin from cyclin dependent kinases 5-knockdown mice

  • Shanshan Yang (College of Animal Science and Technology, Hebei Normal University of Science & Technology) ;
  • Dingxing Jiao (College of Animal Science and Technology, Hebei Normal University of Science & Technology) ;
  • Tao Song (College of Animal Science and Technology, Hebei Normal University of Science & Technology) ;
  • Ping Rui (College of Animal Science and Technology, Hebei Normal University of Science & Technology) ;
  • Ruiwen Fan (College of Veterinary Medicine, Shanxi Agricultural University) ;
  • Zengjun Ma (College of Animal Science and Technology, Hebei Normal University of Science & Technology)
  • 투고 : 2023.07.04
  • 심사 : 2023.10.02
  • 발행 : 2024.04.01

초록

Objective: This study aimed to identify genes regulated by cyclin dependent kinases 5 (CDK5) that participate in hair pigmentation in mice. Methods: The mRNA expression profiles of skin samples from CDK5-knockdown mice were constructed using high-throughput RNA sequencing and compared with those of wild-type mice. Results: In total, 8,002 known genes were differentially expressed between CDK5-knockdown and wild-type mice. Of these, 3,658 were upregulated and 4,344 were downregulated in the skin of CDK5-knockdown mice. An additional 318 previously unknown genes were also differentially expressed, with 171 downregulated and 147 upregulated genes in the skin of CDK5-knockdown mice. Of the known genes expressed in mouse skin, 80 were associated with hair color, with 61 showing lower expression and 19 exhibiting higher expression in skin of CDK5-knockdown mice. Importantly, the expression of the tyrosinase-related protein 1 (TYRP1) and the calcium signaling pathway were also found to be regulated by CDK5, suggesting that pigmentation is regulated by CDK5 via the calcium signaling pathway and TYRP1. Conclusion: The transcriptome profiles obtained from the skin of CDK5-knockdown mice compared to wild-type mice provide a valuable resource to help understand the mechanism by which CDK5 regulates melanogenesis in mice and other animals.

키워드

과제정보

This work was supported by the Scientific Research Fund of the Hebei Normal Unixersity of Science and Technology (Grant number: 2023YB003) and Special Project for Basic Scientific Research of Provincial Universities (Grant number: 2023JK02).

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