Animal Bioscience

  • 제37권4호
  • /
  • Pages.567-575
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  • 2024
  • /
  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Gene expression profiles of skin from cyclin dependent kinases 5-knockdown mice

  • Shanshan Yang (College of Animal Science and Technology, Hebei Normal University of Science & Technology) ;
  • Dingxing Jiao (College of Animal Science and Technology, Hebei Normal University of Science & Technology) ;
  • Tao Song (College of Animal Science and Technology, Hebei Normal University of Science & Technology) ;
  • Ping Rui (College of Animal Science and Technology, Hebei Normal University of Science & Technology) ;
  • Ruiwen Fan (College of Veterinary Medicine, Shanxi Agricultural University) ;
  • Zengjun Ma (College of Animal Science and Technology, Hebei Normal University of Science & Technology)
  • 투고 : 2023.07.04
  • 심사 : 2023.10.02
  • 발행 : 2024.04.01
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초록

Objective: This study aimed to identify genes regulated by cyclin dependent kinases 5 (CDK5) that participate in hair pigmentation in mice. Methods: The mRNA expression profiles of skin samples from CDK5-knockdown mice were constructed using high-throughput RNA sequencing and compared with those of wild-type mice. Results: In total, 8,002 known genes were differentially expressed between CDK5-knockdown and wild-type mice. Of these, 3,658 were upregulated and 4,344 were downregulated in the skin of CDK5-knockdown mice. An additional 318 previously unknown genes were also differentially expressed, with 171 downregulated and 147 upregulated genes in the skin of CDK5-knockdown mice. Of the known genes expressed in mouse skin, 80 were associated with hair color, with 61 showing lower expression and 19 exhibiting higher expression in skin of CDK5-knockdown mice. Importantly, the expression of the tyrosinase-related protein 1 (TYRP1) and the calcium signaling pathway were also found to be regulated by CDK5, suggesting that pigmentation is regulated by CDK5 via the calcium signaling pathway and TYRP1. Conclusion: The transcriptome profiles obtained from the skin of CDK5-knockdown mice compared to wild-type mice provide a valuable resource to help understand the mechanism by which CDK5 regulates melanogenesis in mice and other animals.

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과제정보

This work was supported by the Scientific Research Fund of the Hebei Normal Unixersity of Science and Technology (Grant number: 2023YB003) and Special Project for Basic Scientific Research of Provincial Universities (Grant number: 2023JK02).

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