The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Stem Rot of Pearl Millet Prevalence, Symptomatology, Disease Cycle, Disease Rating Scale and Pathogen Characterization in Pearl Millet-Klebsiella Pathosystem

  • Vinod Kumar Malik (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Pooja Sangwan (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Manjeet Singh (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Pavitra Kumari (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Niharika Shoeran (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Navjeet Ahalawat (Department of Bioinformatics and Computational Biology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Mukesh Kumar (Department of Genetics and Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Harsh Deep (Department of Genetics and Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Kamla Malik (Department of Microbiology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Preety Verma (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Pankaj Yadav (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Sheetal Kumari (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Aakash (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Sambandh Dhal (Department of Electrical and Computer Engineering, Texas A&M University)
  • 투고 : 2023.09.18
  • 심사 : 2023.12.29
  • 발행 : 2024.02.01
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초록

The oldest and most extensively cultivated form of millet, known as pearl millet (Pennisetum glaucum (L.) R. Br. Syn. Pennisetum americanum (L.) Leeke), is raised over 312.00 lakh hectares in Asian and African countries. India is regarded as the significant hotspot for pearl millet diversity. In the Indian state of Haryana, where pearl millet is grown, a new and catastrophic bacterial disease known as stem rot of pearl millet spurred by the bacterium Klebsiella aerogenes (formerly Enterobacter) was first observed during fall 2018. The disease appears in form of small to long streaks on leaves, lesions on stem, and slimy rot appearance of stem. The associated bacterium showed close resemblance to Klebsiella aerogenes that was confirmed by a molecular evaluation based on 16S rDNA and gyrA gene nucleotide sequences. The isolates were also identified to be Klebsiella aerogenes based on biochemical assays, where Klebsiella isolates differed in D-trehalose and succinate alkalisation tests. During fall 2021-2023, the disease has spread all the pearl millet-growing districts of the state, extending up to 70% disease incidence in the affected fields. The disease is causing considering grain as well as fodder losses. The proposed scale, consisting of six levels (0-5), is developed where scores 0, 1, 2, 3, 4, and 5 have been categorized as highly resistant, resistant, moderately resistant, moderately susceptible, susceptible, and highly susceptible disease reaction, respectively. The disease cycle, survival of pathogen, and possible losses have also been studied to understand other features of the disease.

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과제정보

The authors are highly grateful to the Department of Plant Pathology, CCS Haryana Agricultural University, Hisar, Haryana, for providing experimental facilities to carry out this work.

참고문헌

  1. Ajayasree, T. S. and Borkar, S. G. 2018. Pathogenic potentiality of the bacterium of Klebsiella pneumoniae strain Borkar on different plants. J. Appl. Biotechnol. Bioeng. 5:233-235.
  2. All India Coordinated Research Project on Pearl Mellet. 2022. All India Coordinated Research Project on Pearl Millet, Jodhpur, Rajasthan, India. URL http://www.aicpmip.res.in [18 September 2023].
  3. Bock, C. H., Wood, B. W. and Gottwald, T. R. 2013. Pecan scab severity: effects of assessment methods. Plant Dis. 97:675-684. https://doi.org/10.1094/PDIS-07-12-0642-RE
  4. Bouvet, O. M. M., Lenormand, P. and Grimont, P. A. D. 1989. Taxonomy diversity of the D-glucose oxidation pathway in the Enterobacteriaceae. Int. J. Syst. Bacteriol. 39:61-67. https://doi.org/10.1099/00207713-39-1-61
  5. Brisse, S. and Verhoef, J. 2001. Phylogenetic diversity of Klebsiella pneumoniae and Klebsiella oxytoca clinical isolates revealed by randomly amplified polymorphic DNA, gyrA and parC genes sequencing and automated ribotyping. Int. J. Syst. Evol. Microbiol. 51:915-924. https://doi.org/10.1099/00207713-51-3-915
  6. Buddenhagen, I. W. 1965. The relation of plant-pathogenic bacteria to the soil. In: Ecology of soil-borne plant pathogens: prelude to biological control, eds. by K. F. Baker and W. C. Snyder, pp. 269-283. University of California Press, Berkeley, CA, USA.
  7. Chandra, N. S., Srivastava, R. K., Udayashankar, A. C., Lavanya, S. N., Prakash, G., Bishnoi, H. R., Kadvani, D. L., Singh, O. V., Niranjana, S. R., Prakash, H. S. and Satyavathi, C. T. 2017. Magnaporthe blast of pearl millet in India: present status and future prospects. All India Coordinated Research Project on Pearl Millet (Indian Council of Agricultural Research), Mandor, India. 52 pp.
  8. Collins, C. H., Lyne, P. M., Grange, J. M. and Falkinham, J. O. 2004. Collins and Lyne's microbiological methods. 8th ed. Arnold, London, UK. 456 pp.
  9. Crosse, J. E. 1971. Interactions between saprophytic and pathogenic bacteria in plant disease. In: Ecology of leaf surface micro-organisms, eds. by T. F. Preece and C. H. Dickinson, pp. 283-290. Academic Press, New York, USA.
  10. Dickinson, A. B. and Mocquot, G. 2008. Studies on the bacterial flora of the alimentary tract of pigs. I. Enterobacteriaceae and other gram-negative bacteria. J. Appl. Bacteriol. 24:252-284. https://doi.org/10.1111/j.1365-2672.1961.tb00261.x
  11. Forbes, G. A. and Korva, J. T. 1994. The effect of using a Horsfall-Barratt scale on precision and accuracy of visual estimation of potato late blight severity in the field. Plant Pathol. 43:675-682. https://doi.org/10.1111/j.1365-3059.1994.tb01606.x
  12. Gibreel, T. M., Dodgson, A. R., Cheesbrough, J., Bolton, F. J., Fox, A. J. and Upton, M. 2012. High metabolic potential may contribute to the success of ST131 uropathogenic Escherichia coli. J. Clin. Microbiol. 50:3202-3207. https://doi.org/10.1128/JCM.01423-12
  13. Hemalatha, T. M., Shanthipriya, M., Vijay Kumar Naik, D., Bhaskara Reddy, B. V., Gurivi Reddy, M., Madhavilatha, L., Hemanth Kumar, M., Vajantha, B., Sarala, N. V. and Tagore, K. R. 2022. First report of 'Candidatus Phytoplasma aurantifolia' related strain (16SrII-D) associated with stunting, little leaf and phyllody disease of pearl millet from South India. Plant Dis. Advanced online publication. https://doi.org/10.1094/PDIS-04-22-0803-PDN.
  14. Holt, J. G., Krieg, N. R., Sneath, P. H. A., Stanley, J. T. and Williams, S. T. 1994. Bergey's manual of determinative bacteriology. 9th ed. Lippincott Williams & Wilkins, Baltimore, MD, USA. 763 pp.
  15. Hormaeche, E. and Edwards, P. R. 1960. A proposed genus Enterobacter. Int. Bull. Bacteriol. Nomencl. Taxon. 10:71-74. https://doi.org/10.1099/0096266X-10-2-71
  16. Huang, M., Lin, L., Wu, Y.-X., Honhing, H., He, P.-F., Li, G.-Z., He, P.-B., Xiong, G.-R., Yuan, Y. and He, Y.-Q. 2016. Pathogenicity of Klebsiella pneumonia (KpC4) infecting maize and mice. J. Integr. Agric. 15:1510-1520. https://doi.org/10.1016/S2095-3119(16)61334-5
  17. Janse, J. D. 2005. Phytobacteriology: principles and practices. CABI Publishing, Cambridge, MA, USA. 366 pp.
  18. Malik, V. K., Sangwan, P., Singh, M., Punia, R., Yadav, D. V., Kumari, P. and Pahuja, S. K. 2022. First report of Klebsiella aerogenes inciting stem rot of pearl millet in Haryana, India. Plant Dis. 106:754.
  19. Malik, V. K., Singh, M., Sangwan, P., Kumari, P., Sharma, B. L., Kumari, P., Verma, P., Yadav, P., Sheoran, N., Singh, A., Singh, D. P., Arya, S. K., Ahalawat, N. and Malik, J. 2023. First report of Klebsiella leaf streak on sorghum caused by Klebsiella variicola in Haryana, India. Plant Dis. 107:2215.
  20. Ministry of Agriculture, Government of India. 2022. Selected state-wise area, production and productivity of Bajra in India (2021-2022). URL https://www.indiastat.com/table/agriculture/selected-state-wise-area-production-productivityb/1440259 [18 September 2023].
  21. Mushineni, A., Balamurugan, A. Shashikumara, P., Pandey, N., Agarwal, D. K., Tarasatyavathi, C., Varshney, R. K. and Nayaka, C. S. 2021. First report of pearl millet bacterial leaf blight caused by Pantoea stewartii subspecies indologenes in India. Plant Dis. 105:3736.
  22. Orskov, I. 1995. The biochemical properties of Klebsiella (Klebsiella aerogenes) strains. Acta Pathol. Microbiol. Scand. 37:353-368. https://doi.org/10.1111/j.1699-0463.1955.tb00958.x
  23. Tamura, K., Stecher, G. and Kumar, S. 2021. MEGA11: Molecular Evolutionary Genetics Analysis version 11 for bigger datasets. Mol. Biol. Evol. 38:3022-3027. https://doi.org/10.1093/molbev/msab120
  24. Tindall, B. J., Sutton, G. and Garrity, G. M. 2017. Enterobacter aerogenes Hormaeche and Edwards 1960 (Approved Lists 1980) and Klebsiella mobilis Bascomb et al. 1971 (Approved Lists 1980) share the same nomenclatural type (ATCC 13048) on the Approved Lists and are homotypic synonyms, with consequences for the name Klebsiella mobilis Bascomb et al. 1971 (Approved Lists 1980). Int. J. Syst. Evol. Microbiol. 67:502-504. https://doi.org/10.1099/ijsem.0.001572
  25. Wesevich, A., Sutton, G., Fouts, D., Fowler, V. G. and Thaden, J. 2019. Newly-named Klebsiella aerogenes is associated with poor clinical outcomes relative to Enterobacter cloacae complex in patients with bloodstream infection. Open Forum Infect. Dis. 6:S129.
  26. Yadav, O. P., Rai, K. N and Gupta, S. K. 2012. Pearl millet: genetic improvement for tolerance to abiotic stresses. In: Improving crop productivity in sustainable agriculture, eds. by N. Tuteja, S. S. Gill and R. Tuteja, pp. 261-288. Wiley-VCH Berlag GmbH & Co., Weinheim, Germany.