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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Recent advances in genome engineering by CRISPR technology

  • Youngsik Lee (Department of Life Science, Chung-Ang University) ;
  • Yeounsun Oh (Department of Life Science, Chung-Ang University) ;
  • Seung Hwan Lee (Department of Life Science, Chung-Ang University)
  • Received : 2023.08.27
  • Accepted : 2023.09.27
  • Published : 2024.01.31
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Abstract

Due to the development of CRISPR technology, the era of effective editing of target genes has arrived. However, the off-target problem that occurs when recognizing target DNA due to the inherent nature of CRISPR components remains the biggest task to be overcome in the future. In this review, the principle of inducing such unintended off-target editing is analyzed from the structural aspect of CRISPR, and the methodology that has been developed to reduce off-target editing until now is summarized.

Keywords

Acknowledgement

This research was supported by grants from the National Research Foundation funded by the Korean Ministry of Education, Science and Technology (NRF-2022R1A2C4001609) and the Korean Fund for Regenerative Medicine (KFRM) grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Health & Welfare) (22A0203L1). This research was also supported by the Chung-Ang University Graduate Research Scholarship in 2023.

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