The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Screening for Mucosal Protective Effects of Various Korean Herbal Medicine Extracts in Gastroesophageal Reflux Disease

한방 추출물의 역류성 식도염 점막보호 효과에 대한 스크리닝

  • Il-ha Jeong (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Min Ju Kim (Research Center for Herbal Convergence on Liver Disease, Daegu Haany University) ;
  • Mi-Rae Shin (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Seong-Soo Roh (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 정일하 (대구한의대학교 한의학과 본초약리학교실) ;
  • 김민주 (대구한의대학교 간질환한약융복합활용연구센터) ;
  • 신미래 (대구한의대학교 한의학과 본초약리학교실) ;
  • 노성수 (대구한의대학교 한의학과 본초약리학교실)
  • Received : 2023.12.11
  • Accepted : 2024.01.25
  • Published : 2024.01.30
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Abstract

Objectives : This study evaluates how various traditional Korean herbal medicines assess MUC5AC expression for esophageal mucosal defense and analyzes the associated mechanisms involved in inflammation. Methods : Forty types of traditional Korean herbal medicines were assessed for in vitro antioxidant activities, and the real-time PCR method was employed to analyze MUC5AC expression under pH 4.5 conditions in human esophageal epithelial cells (HET-1A). Eight types of Korean herbal medicines were evaluated for in vitro antioxidant activities, and Reactive oxygen specise (ROS) expression was analyzed under bile salt (480 𝜇M) and pH 5.5 conditions in human esophageal epithelial cells (HET-1A). Simulation experiments involving bile salts and acidity were conducted for one hour to assess the efficacy of four drugs, and the activities of Mitogen-activated Protein Kinase (MEK), Nuclear Factor Kappa B (NF-𝜅B), and Cyclooxygenase-2 (COX-2) were detected through Western blot analysis. Results : Compared to the Normal group, the Control group exhibited higher ROS generation. Such increased ROS levels were significantly reduced by four extracts: Citrus Unshius Pericarpium (CUP), Cnidium officinale Rhizoma (CR), Ginseng Radix (GR), and Linderae Radix (LR). The protein expression of COX-2 decreased with the treatment of LR, CUP, and CR. Particularly, CUP and CR exhibited superior effects compared to other groups in inhibiting the phosphorylation of NF-𝜅B. Conclusion : Based on the results obtained, we have identified drugs that inhibit oxidative stress and inflammation caused by bile acid in esophageal epithelial cells. Our future plans involve comparing and analyzing the efficacy of these herbal medicines through animal experiments.

Keywords

Acknowledgement

이 성과물은 정부 (과학기술정보통신부)의 재원으로 한국연구재단 (No.2022R1A2B5B0100257112) 및 여성과학공학기술인 R&B 경력복귀 지원사업으로 한국연구재단과 한국여성과학기술인지원센터의 지원을 받아 연구되었습니다.

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