The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The Anti-Obesity Activity of Syzygium aromaticum L. in High-Fat Diet-induced Obese Mice

고지방식이로 유도된 비만 마우스에서 정향(丁香)의 항비만 효과

  • Hui Yeon An (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Seong-Soo Roh (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Mi-Rae Shin (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 안희연 (대구한의대학교 한의학과 본초약리학교실) ;
  • 노성수 (대구한의대학교 한의학과 본초약리학교실) ;
  • 신미래 (대구한의대학교 한의학과 본초약리학교실)
  • Received : 2023.12.08
  • Accepted : 2024.01.25
  • Published : 2024.01.30
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Abstract

Objectives : This study aims to analyze the anti-obesity effect of Syzygium aromaticum L. (SA) in obese mice made by a 60% high-fat diet (HFD). Methods : The antioxidant activities of SA were evaluated in vitro. To assess the anti-obesity effect of SA, male C57BL/6 mice were divided into five groups: Normal, Control, GC100 (Garcinia cambogia 100 mg/kg/day), SA100 (SA 100 mg/kg/day), SA200 (SA 200 mg/kg/day). All groups underwent a 6-week regimen of HFD and oral administration, except for the Normal group. Subsequently, we performed blood analysis, western blotting, and histopathological staining. Results : SA demonstrated effectiveness in antioxidant measurements. SA treatment resulted in a significant decrease in body weight gain, along with reductions in liver and epididymal fat weights. Serum triglyceride (TG), total cholesterol (TC), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and leptin levels were reduced with SA treatment. Moreover, in the SA100 group, the reduction of both TG and TC synthesis was caused by inhibiting the sterol regulatory element-binding transcription factor 1 (SREBP-1) and sterol regulatory element-binding transcription factor 2 (SREBP-2) through the Sirtuin 1 (Sirt1)/phospho-AMP-activated protein kinase (p-AMPK) pathway. Furthermore, SA treatment at a dose of 100 mg/kg reduced the accumulation of lipid droplets in the liver and the adipocyte size of the epididymal fat. Conclusion : Our research reveals the anti-obesity effects of SA by demonstrating its ability to inhibit body weight gain and lipid accumulation, suggesting that SA might be promising for obesity treatment.

Keywords

Acknowledgement

이 성과물은 정부 (과학기술정보통신부, No.2018R1A5A2025272; 교육부, 2021R1I1A1A01059605)의 재원으로 한국연구재단의 지원을 받아 수행되었습니다.

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