Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Age-induced Changes in Ginsenoside Accumulation and Primary Metabolic Characteristics of Panax Ginseng in Transplantation Mode

  • Wei Yuan (Liaoning University of Traditional Chinese Medicine) ;
  • Qing-feng Wang (Liaoning University of Traditional Chinese Medicine) ;
  • Wen-han Pei (Macau University of Science and Technology) ;
  • Si-yu Li (Liaoning University of Traditional Chinese Medicine) ;
  • Tian-min Wang (Liaoning University of Traditional Chinese Medicine) ;
  • Hui-peng Song (Liaoning University of Traditional Chinese Medicine) ;
  • Dan Teng (He University) ;
  • Ting-guo Kang (Liaoning University of Traditional Chinese Medicine) ;
  • Hui Zhang (Liaoning University of Traditional Chinese Medicine)
  • Received : 2023.02.04
  • Accepted : 2023.09.25
  • Published : 2024.01.01
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Abstract

Background: Ginseng (Panax ginseng Mayer) is an important natural medicine. However, a long culture period and challenging quality control requirements limit its further use. Although artificial cultivation can yield a sustainable medicinal supply, research on the association between the transplantation and chaining of metabolic networks, especially the regulation of ginsenoside biosynthetic pathways, is limited. Methods: Herein, we performed Liquid chromatography tandem mass spectrometry based metabolomic measurements to evaluate ginsenoside accumulation and categorise differentially abundant metabolites (DAMs). Transcriptome measurements using an Illumina Platform were then conducted to probe the landscape of genetic alterations in ginseng at various ages in transplantation mode. Using pathway data and crosstalk DAMs obtained by MapMan, we constructed a metabolic profile of transplantation Ginseng. Results: Accumulation of active ingredients was not obvious during the first 4 years (in the field), but following transplantation, the ginsenoside content increased significantly from 6-8 years (in the wild). Glycerolipid metabolism and Glycerophospholipid metabolism were the most significant metabolic pathways, as Lipids and lipid-like molecule affected the yield of ginsenosides. Starch and sucrose were the most active metabolic pathways during transplantation Ginseng growth. Conclusion: This study expands our understanding of metabolic network features and the accumulation of specific compounds during different growth stages of this perennial herbaceous plant when growing in transplantation mode. The findings provide a basis for selecting the optimal transplanting time.

Keywords

Acknowledgement

This work was supported by the National Nature Science Foundation of China (82173935). This work was also supported by the Inner Mongolia Major Science and Technology Project (2021SZD0030).

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