Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Comparative analysis of liver transcriptome reveals adaptive responses to hypoxia environmental condition in Tibetan chicken

  • Yongqing Cao (College of Animal Science and Technology, Gansu Agricultural University) ;
  • Tao Zeng (State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Science) ;
  • Wei Han (Jiangsu Institute of Poultry Science) ;
  • Xueying Ma (Institute of Animal Husbandry and Veterinary Medicine, Tibet Academy Agricultural and Animal Husbandry Sciences) ;
  • Tiantian Gu (State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Science) ;
  • Li Chen (State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Science) ;
  • Yong Tian (State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Science) ;
  • Wenwu Xu (State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Science) ;
  • Jianmei Yin (Jiangsu Institute of Poultry Science) ;
  • Guohui Li (Jiangsu Institute of Poultry Science) ;
  • Lizhi Lu (State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Science) ;
  • Shuangbao Gun (College of Animal Science and Technology, Gansu Agricultural University)
  • Received : 2023.04.05
  • Accepted : 2023.07.11
  • Published : 2024.01.01
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Abstract

Objective: Tibetan chickens, which have unique adaptations to extreme high-altitude environments, exhibit phenotypic and physiological characteristics that are distinct from those of lowland chickens. However, the mechanisms underlying hypoxic adaptation in the liver of chickens remain unknown. Methods: RNA-sequencing (RNA-Seq) technology was used to assess the differentially expressed genes (DEGs) involved in hypoxia adaptation in highland chickens (native Tibetan chicken [HT]) and lowland chickens (Langshan chicken [LS], Beijing You chicken [BJ], Qingyuan Partridge chicken [QY], and Chahua chicken [CH]). Results: A total of 352 co-DEGs were specifically screened between HT and four native lowland chicken breeds. Gene ontology and Kyoto encyclopedia of genes and genomes enrichment analyses indicated that these co-DEGs were widely involved in lipid metabolism processes, such as the peroxisome proliferator-activated receptors (PPAR) signaling pathway, fatty acid degradation, fatty acid metabolism and fatty acid biosynthesis. To further determine the relationship from the 352 co-DEGs, protein-protein interaction network was carried out and identified eight genes (ACSL1, CPT1A, ACOX1, PPARC1A, SCD, ACSBG2, ACACA, and FASN) as the potential regulating genes that are responsible for the altitude difference between the HT and other four lowland chicken breeds. Conclusion: This study provides novel insights into the molecular mechanisms regulating hypoxia adaptation via lipid metabolism in Tibetan chickens and other highland animals.

Keywords

Acknowledgement

This research was funded by the National Key Research and Development Program of China (2021YFD1200302), Project of Key Research and Development Plan (Modern Agriculture) of Jiangsu Province (BE2019353), "JBGS" Project of Seed Industry Revitalization in Jiangsu Province (JBGS [2021] 029), and the Key Research and Development Program of Zhejiang Province (2021C02034).

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