Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
권호 표지
DOI QR코드

DOI QR Code

Isolation and characterization of two unreported yeast species from wildflowers and mountain soil in Korea in the families Filobasidiaceae and Piskurozymaceae

  • Seona Park (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Sathiyaraj Srinivasan (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
  • Received : 2024.08.20
  • Accepted : 2024.09.11
  • Published : 2024.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to isolate and identify wild yeasts from wildflower samples in Okcheongun and soil samples in Nowon-gu, Republic of Korea. Out of the fourteen strains identified, twelve had been previously reported, whereas two strains were unreported in the Republic of Korea. Identification of wild yeast strains was performed through pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene using the Basic Local Alignment Search Tool (BLAST) (Fell et al., 2000). Cell morphology was examined using a phase contrast microscope, and assimilation tests were conducted with the API 20C AUX kit. All strains were classified under the phylum Basidiomycota. The two unreported yeast strains, MFL-1 and NYB3-16, are classified under the genus Naganishia (family Filobasidiaceae, order Filobasidiales, class Tremellomycetes) and the genus Solicoccozyma (family Piskurozymaceae, order Filobasidiales, class Tremellomycetes), respectively. These two strains exhibited morphologies consistent with their type species. MFL-1 cells were circular, while NYB3-16 cells were oval. Both strains demonstrated polar budding. This study highlighted the morphological and biochemical characteristics of the two unreported yeast species that had not been previously documented in Korea.

Keywords

Acknowledgement

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment(MOE) of the Republic of Korea (NIBRE202403 and NIBR202402104) and was also supported by Seoul Women's University (2024).

References

  1. Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25(17):3389-3402.
  2. Boekhout, T., A.S. Amend, F. El Baidouri, T. Gabaldon, J. Geml, M. Mittelbach, V. Robert, C.S. Tan, B. Turchetti, D. Vu, Q.-M. Wang and Yurkov, A. 2022. Trends in yeast diversity discovery. Fungal Divers. 114(1):491-537.
  3. Farias, G.S., J.A. Santos, P. Giovanella and L.D. Sette. 2022. Antarctic-derived yeasts: taxonomic identification and resistance to adverse conditions. An. Acad. Bras. Cienc. 94:e20210592.
  4. Fell, J.W., T. Boekhout, A. Fonseca and J.P. Sampaio. 2001. Basidiomycetous yeasts. Systematics and Evolution, 3-35.
  5. Fell, J.W., T. Boekhout, A. Fonseca, G. Scorzetti and A. Statzell-Tallman. 2000. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int. J. Syst. Evol. Microbiol. 50(3):1351-1371.
  6. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4):783-791.
  7. Fonseca, A., T. Boekhout and J.W. Fell. 2011. Cryptococcus Vuillemin (1901). In: The yeasts(pp. 1661-1737). Elsevier.
  8. Guarro, J., J. Gene and A.M. Stchigel. 1999. Developments in fungal taxonomy. Clin. Microbiol. Rev. 12(3):454-500.
  9. Kim, J.H., N.M. Kim and J.S. Lee. 1999. Physiological characteristics and ethanol fermentation of thermotolerant yeast Saccharomyces cerevisiae OE-16 from traditional Meju. Korean J. Food Nutr. 12: 490-495.
  10. Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press.
  11. Kurtzman, C.P. and C.J. Robnett. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73(4):331-371.
  12. Lee, J.S., S.H. Yi, S.J. Kwon, C. Ahn and J.Y. Yoo. 1997. Enzyme activities and physiological functionality of yeasts from traditional Meju. Korean J. Appl. Microbiol. Biotechnol. 25(5):448-453.
  13. Li, A.H., Y. Zhou, B.S. Jia, Z.X. Liu, J.P. Sampaio and Y.G. Zhou. 2019. Heterocephalacria sinensis sp. nov., Phaeotremella lacus sp. nov. and Solicoccozyma aquatica sp. nov., three novel basidiomycetous yeast species isolated from crater lakes. Int. J. Syst. Evol. Microbiol. 69(12):3728-3739.
  14. Liu, X.Z., Q.M. Wang, M. Goker, M. Groenewald, A.V. Kachalkin, H.T. Lumbsch, A.M. Millanes, M. Wedin, A.M. Yurkov, T. Boekhout and F.Y. Bai. 2015. Towards an integrated phylogenetic classification of the Tremellomycetes. Studies in Mycology 81(1):85-147.
  15. Luo, B., H. Sun, Y. Zhang, Y. Gu, W. Yan, R. Zhang and Y. Ni. 2019. Habitat-specificity and diversity of culturable cold-adapted yeasts of a cold-based glacier in the Tianshan Mountains, northwestern China. App. Microbiol. Biotechnol. 103:2311-2327.
  16. Maeng, S., Y. Park, G.H. Sung, H.B. Lee, M.K. Kim and S. Srinivasan. 2022. Description of Vishniacozyma terrae sp. nov. and Dioszegia terrae sp. nov., Two Novel Basidiomycetous Yeast Species Isolated from Soil in Korea. Mycobiology 50(6):439-447.
  17. Nizovoy, P., N. Bellora, S. Haridas, H. Sun, C. Daum, K. Barry, I.V. Grigoriev, D. Libkind, L.B. Connell and M. Moline. 2021. Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica. FEMS Yeast Research 21(1):foaa056.
  18. Park, Y., S. Maeng and S. Srinivasan. 2011. Isolation and characterization of four unrecorded wild yeasts from the soils of Republic of Korea in winter. Journal of Species Research 12(3):197-202.
  19. Park, Y., S. Maeng, J. Oh, G.H. Sung and S. Srinivasan. 2021. Mrakia terrae sp. nov. and Mrakia soli sp. nov., two novel basidiomycetous yeast species isolated from soil in Korea. Mycobiology 49(5):469-475.
  20. Passoth, V. 2017. Lipids of yeasts and filamentous fungi and their importance for biotechnology. Biotechnology of Yeasts and Filamentous Fungi, 149-204.
  21. Rainey, F. and A. Oren. 2011. Taxonomy of prokaryotes. Academic Press.
  22. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4(4):406-425.
  23. Seok, J., J. Bai and S. Srinivasan, 2024. Isolation and characterization of two unrecorded yeast species in the phylum Basidiomycota. Journal of Species Research 13(1):105-110.
  24. Tamura, K., G. Stecher and S. Kumar. 2021. MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol. Biol. Evol. 38:3022-3027.