Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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Characterization of two unrecorded yeast species, Starmerella apicola and Symmetrospora symmetrica, isolated from Apis mellifera and Citrus sunki in Republic of Korea

  • Eo Jin Kim (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Myung Kyum Kim (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
  • Received : 2024.07.15
  • Accepted : 2024.09.10
  • Published : 2024.11.30
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Abstract

The purpose of this study is to isolate and identify wild yeasts from Apis mellifera and Citrus sunki samples collected in Okcheon-gun and Jeju-si, Republic of Korea. Among ten strains, one strain had been previously reported, but nine strains were unreported in the Republic of Korea. To identify the wild yeast strains, pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene sequence were conducted using the Basic Local Alignment Search Tool (BLAST) (Fell et al., 2000). Cell morphologies were observed using a phase contrast microscope, and assimilation tests were performed using the API 20C AUX kit. The two unrecorded yeast strains, MBEE-1 and MMD-1, belong to the genus Starmerella (family Saccharomycetales incertae sedis, Order Saccharomycetales, Class Saccharomycetes) and the genus Symmetrospora (family Symmetrosporaceae, Order Cystobasidiomycetes incertae sedis, Class Cystobasidiomycetes), respectively. Both strains had oval-shaped and polar budding cells. This research elucidated the morphological and biochemical properties of these two previously unreported yeast species in Korea.

Keywords

Acknowledgement

This work was supported by a grant from the National Institute of Biological Resources(NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBRE202406 and NIBR202402104) and was also supported by Seoul Women's University (2024).

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