Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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Report of five unrecorded bacterial species in Korea belonging to the genus Bradyrhizobium

  • Hyorim Choi (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Yiseul Kim (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Jun Heo (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration)
  • Received : 2024.07.09
  • Accepted : 2024.10.24
  • Published : 2024.11.30
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Abstract

Symbiotic bacteria belonging to the genus Bradyrhizobium were generally called 'rhizobia' which can fix nitrogen by nodulating legumes. Between 2000 and 2001, numerous Bradyrhizobium strains were isolated from root nodules. However, due to challenges in identification, many of these isolates remained unreported. Recent advances in phylogenetic analyses based on whole genome sequencing have resolved these difficulties in species identification. Consequently, five of these strains have now been re-identified and are described as previously unrecorded species in the Republic of Korea. As a result, we report Bradyrhizobium elkanii Glm-3 (=KACC 10989), Bradyrhizobium australafricanum Glm-4 (=KACC 10990), Bradyrhizobium huanghuaihaiense Glm-7 (=KACC 10993), these were isolated from root nodules of Glycine max, Bradyrhizobium frederickii Kus-5 (=KACC 11016) isolated from root nodules of Kummerowia striata and Bradyrhizobium diazoefficiens Leb-14 (=KACC 11026) isolated from root nodules of Lespedeza bicolor.

Keywords

Acknowledgement

This study was carried out with the support(PJ017286) of National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

References

  1. Delamuta, J.R.M., R.A. Ribeiro, E. Ormeno-Orrillo, I.S. Melo, E. Martinez-Romero and M. Hungria. 2013. Polyphasic evidence supporting the reclassification of Bradyrhizobium japonicum group Ia strains as Bradyrhizobium diazoefficiens sp. nov. International Journal Systematic and Evolutionary Microbiology 63:3342-3351.
  2. Felsenstein, J. 1981. Evolutionary trees from DNA sequences: A maximum likelihood approach. Journal of Molecular Evolution 17:368-376.
  3. Felsenstein, J. 1985. Confidence Limits on Phylogenies: An Approach Using the Bootstrap. Evolution 39:783-791.
  4. Fitch, W.M. 1971. Toward defining the course of evolution: minimum change for a specific tree topology. Systematic Biology 20:406-416.
  5. Jordan, D. 1982. Transfer of Rhizobium japonicum Buchanan 1980 to Bradyrhizobium gen. nov., a genus of slow-growing, root nodule bacteria from leguminous plants. International Journal Systematic and Evolutionary Microbiology 32:136-139.
  6. Kim, Y.-e., H. Shin, Y. Yang and H.-G. Hur. 2022. Geographical distribution and genetic diversity of Bradyrhizobium spp. isolated from Korean soybean root nodules. Applied Biological Chemistry 65:39.
  7. Kwon, S.W., J.Y. Park, J.S. Kim, J.W. Kang, Y.H. Cho, C.K. Lim and G.B. Lee. 2005. Phylogenetic analysis of the genera Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium on the basis of 16S rRNA gene and internally transcribed spacer region sequences. International Journal of Systematic and Evolutionary Microbiology 55:263-270.
  8. Parker, M.A. 2015. The spread of Bradyrhizobium lineages across host legume clades: from Abarema to Zygia. Microbial Ecology 69:630-640.
  9. Qian, J., S-.W. Kwon and M.A. Parker. 2003. rRNA and nifD phylogeny of Bradyrhizobium from sites across the Pacific Basin. FEMS Microbiology Letters 219:159-165.
  10. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
  11. Stepkowski, T., M. Lionel, K. Agnieszka, M. Alison, J.L. Ian and H. John. 2005. European Origin of Bradyrhizobium Populations Infecting Lupins and Serradella in Soils of Western Australia and South Africa. Applied and Environmental Microbiology 71:7041-7052.
  12. Tamura, K., G. Stecher and S. Kumar. 2021. MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution 38:3022-3027.
  13. Tao, J., S. Wang, T. Liao and H. Luo. 2021. Evolutionary origin and ecological implication of a unique nif island in free-living Bradyrhizobium lineages. The ISME Journal 15:3195-3206.
  14. VanInsberghe, D., K.R. Maas, E. Cardenas, C.R. Strachan, S.J. Hallam and W.W. Mohn. 2015. Non-symbiotic Bradyrhizobium ecotypes dominate North American forest soils. The ISME Journal 9:2435-2441.
  15. Vinuesa, P., M. Leon-Barrios, C. Silva, A. Willems, A. Jarabo-Lorenzo, R. Perez-Galdona, D. Werner and E. Martinez-Romero. 2005. Bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies alpha and Bradyrhizobium genospecies beta. International Journal Systematic and Evolutionary Microbiology 55:569-575.