The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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Anti-inflammatory Effect of Baekho-tang Extract through Endocannabinoid system (ECS) Control in Atopic Dermatitis

아토피피부염에서 Endocannabinoid system (ECS) 조절을 통한 백호탕 추출물의 염증 완화 효과

  • Ahn Sang Hyun (Dept. of anatomy, College of Korean Medicine, Semyung University) ;
  • Kim Ki Bong (School of Korean Medicine, Pusan National University ) ;
  • Jeong Aram (Dept. of Pediatrics, College of Korean Medicine, Gachon University)
  • 안상현 (세명대학교 한의과대학 해부학교실) ;
  • 김기봉 (부산대학교 한의학전문대학원 한방소아과학교실) ;
  • 정아람 (가천대학교 한의과대학 소아과학교실)
  • Received : 2023.10.31
  • Accepted : 2023.11.24
  • Published : 2023.11.30
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Abstract

Objectives The aim of this study was to identify the effect of Baekho-tang extract on epidermal barrier recovery and inflammation relief in atopic dermatitis-induced mice through Endocanabinoid system (ECS) regulation. Methods In this study, we used 4-week-old NC/Nga mice were divided into 4 group: lipid barrier elimination group (LBEG), palmitoylethanolamide treated group after lipid barrier elimination (PEAT), Baekho-tang extract treatment group after lipid barrier elimination (BHTT) and control group (Ctrl). Each group was assigned 10 animals. We identified that cannabinoid receptor (CB) 1, CB2, CD (Cluster of Differentiation) 68, inducible nitric oxide synthase (iNOS), substance P and Matrix metallopeptidase 9 (MMP-9) through our immunohistochemistry. Results We discovered that when compared to PEAT, 8-hydroxydeoxyguanosine, a marker of oxidative stress in the epidermal barrier, and CB1 and CB2, markers of ECS modulation, were less activated in BHTT. These results led to an anti-inflammatory response in BHTT, with a significant decrease in several inflammatory mediators such as CD 68, iNOS, substance P and MMP-9 compared to PEAT and LBEG. Conclusions These results suggest that the Baekho-tang extract can reduce the inflammation of atopic dermatitis by restoring the structural damage of the skin lipid barrier through ECS modulation.

Keywords

Acknowledgement

이 성과는 정부 (과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임 (No. 2020R1F1A1061970).

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