Journal of the Korean Society of Visualization (한국가시화정보학회지)

The Korean Society of Visualization (한국가시화정보학회)
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Development of a Total Internal Reflection Fluorescence (TIRF) Microscopy for Precise Imaging the Drying Pattern of a Sessile Droplet

고착 액적 증발면의 정밀 관측을 위한 전반사 형광 현미경 기법 개발

  • Wonho Cho (School of Mechanical Engineering, Sungkyunkwan University) ;
  • Jinkee Lee (Institute of Quantum Biophysics, Sungkyunkwan University)
  • Received : 2023.10.30
  • Accepted : 2023.11.21
  • Published : 2023.11.30
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Abstract

Compared to epifluorescence(EPI) microscopy which captures fluorescence from the entire depth of sample, total internal reflection fluorescence(TIRF) can selectively visualize only a single surface of it. TIRF uses a thin evanescent field generated by the total internal reflection of laser light on surface. However, conventional TIRF system are designed for total internal reflection to occur at the upper surface of sample, making them unsuitable for sessile droplet imaging. We designed a TIRF system suitable for a sessile droplet imaging by utilizing slide glass as a lightguide. We presented the details for constructing the TIRF system using a prism, slide glass, air slit, and optical trap. Then, we compared the TIRF with EPI by imaging the droplet with fluorescent particles during its drying process. As a result, TIRF allows us to distinctly visualize the drying pattern on the bottom surface of droplet.

Keywords

Acknowledgement

본 연구는 정부(교육부 및 미래창조과학부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임 (No. NRF2021R1A6A1A03039696, No. 2020R1A2C3010568).

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