Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Cudrania tricuspidata Leaf and Achyranthes japonica Nakai Complex in MIA-Induced Osteoarthritis Rats

꾸지뽕나무 잎과 우슬 복합물이 MIA로 골관절염이 유도된 Rat에 미치는 영향

  • Kil-Ho Cheong (Department of Pathology, College of Korean Medicine, Daejeon University) ;
  • In-Hwan Joo (Department of Pathology, College of Korean Medicine, Daejeon University) ;
  • Dong-Hee Kim (Department of Pathology, College of Korean Medicine, Daejeon University)
  • 정길호 (대전대학교 한의과대학 병리학교실) ;
  • 주인환 (대전대학교 한의과대학 병리학교실) ;
  • 김동희 (대전대학교 한의과대학 병리학교실)
  • Received : 2023.04.28
  • Accepted : 2023.06.25
  • Published : 2023.06.25
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Abstract

This study was to investigate the effects of cudrania tricuspidata leaf and achyranthes japonica nakai complex (CAC) treatment on monosodium iodoacetate (MIA)-induced osteoarthritis rats. Osteoarthritis was induced by injection of MIA (3 mg) into right knee joints of rats. The rats were divided into 5 groups; Non-induced normal rat (Con, n=10), MIA-induced osteoarthritis rat (MIA, n=10), osteoarthritis rat treated with indomethacin 2 mg/kg (Indo, n=10), osteoarthritis rat treated with CAC 200 mg/kg (Low, n=10) or 400 mg/kg (High, n=10). The rats were treated orally for 14 days. On the last day of oral administration, the hind paw weight bearing of the experimental animals was measured using an incapacitance test meter. The interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), prostaglandin E2 (PGE2), cartilage oligomeric matrix protein (COMP) and, C-telopeptides of type II collagen (CTX-II) levels in serum were measured by ELISA. And Then, that gene expression (IL-1β, IL-6, TNF-α, COX-2) levels in cartilage were measured by qRT-PCR. Also, cartilage volume was measured by micro CT arthrography. Administration of CAC reduced the inflammatory cytokine (IL-1β, IL-6, TNF-α, PGE2) levels in serum and gene expression levels in cartilage, and the COMP and CTX-II level in serum, which is an indicator of cartilage degradation. In addition, the hind paw weight bearing and cartilage volume, which were decreased due to osteoarthritis, were significantly increased. In the future, if the results of clinical studies including studies on safety are supplemented, it is considered that it can be used as an oriental medicine treatment to improve or treat symptoms of osteoarthritis.

Keywords

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