Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Development of Biosensors for Rapid Detection of Foodborne Pathogenic Bacteria using CRISPR/Cas

CRISPR/Cas 시스템 기술을 활용한 고위험성 식중독 세균 신속 검출을 위한 바이오센서 개발

  • Seon Yeong Jo (Department of Food Science and Technology, Chung-Ang University) ;
  • Jong Pil Park (Department of Food Science and Technology, Chung-Ang University)
  • Received : 2023.07.25
  • Accepted : 2023.08.11
  • Published : 2023.10.30
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Abstract

Rapid and accurate detection of pathogenic bacteria is crucial for various applications, including public health and food safety. However, existing bacteria detection techniques have several drawbacks as they are inconvenient and require time-consuming procedures and complex machinery. Recently, the precision and versatility of CRISPR/Cas system has been leveraged to design biosensors that offer a more efficient and accurate approach to bacterial detection compared to the existing techniques. Significant research has been focused on developing biosensors based on the CRISPR/Cas system which has shown promise in efficiently detecting pathogenic bacteria or virus. In this review, we present a biosensor based on the CRISPR/Cas system that has been specifically developed to overcome these limitations and detect different pathogenic bacteria effectively including Vibrio parahaemolyticus, Salmonella, E. coli O157:H7, and Listeria monocytogenes. This biosensor takes advantage of the CRISPR/Cas system's precision and versatility for more efficiently accurately detecting bacteria compared to the previous techniques. The biosensor has potential to enhance public health and ensure food safety as the biosensor's design can revolutionize method of detecting pathogenic bacteria. It provides a rapid and reliable method for identifying harmful bacteria and it can aid in early intervention and preventive measures, mitigating the risk of bacterial outbreaks and their associated consequences. Further research and development in this area will lead to development of even more advanced biosensors capable of detecting an even broader range of bacterial pathogens, thereby significantly benefiting various industries and helping in safeguard human health

Keywords

Acknowledgement

이 연구는 2023년 식품의약품안전처 지원 과제(21153MFDS605)에 의해 수행됐으며, 이에 감사드립니다.

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