Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Isolation of Peripheral Blood-Derived Mesenchymal Stem Cells in Mares and Foals

  • Ye-Eun Oh (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Eun-Bee Lee (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Jong-Pil Seo (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
  • Received : 2023.06.30
  • Accepted : 2023.09.18
  • Published : 2023.10.31
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Abstract

Peripheral blood-derived mesenchymal stem cells (PB-MSCs) have shown promise in cell-based therapy, as they can be harvested with ease through minimally invasive procedures. This study aimed to isolate PB-MSCs from foals and mares and to compare the proliferation and cellular characteristics of the PB-MSCs between the two groups. Six pairs of mares and their foals were used in this study. MSCs were isolated from PB by direct plating in a tissue culture medium, and cell proliferation (population doubling time [PDT], and colony-forming unit-fibroblast assay [CFU-F]), and characterization (morphology, plastic adhesiveness, colony formation, trilineage differentiation) were examined. There was no significant difference in the PB-MSC yield, CFU-F, and PDT between the mares and foals. PB-MSCs from both mares and foals showed typical MSC characteristics in terms of spindle-shaped morphology, plastic adhesive properties, formation of colonies, trilineage differentiation. These results suggest that PB-MSCs isolated from horses, both adult horses, and foals, can be used for equine cell-based therapy.

Keywords

Acknowledgement

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Republic of Korea (grant number NRF-2017R1C1B1006030).

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