Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Genetic diversity analysis of the line-breeding Hanwoo population using 11 microsatellite markers

  • Shil Jin (Hanwoo Research Institute, National Institute of Animal Science) ;
  • Jeong Il Won (Hanwoo Research Institute, National Institute of Animal Science) ;
  • Byoungho Park (Animal Breeding & Genetics Division, National Institute of Animal Science) ;
  • Sung Woo Kim (Hanwoo Research Institute, National Institute of Animal Science) ;
  • Ui Hyung Kim (Hanwoo Research Institute, National Institute of Animal Science) ;
  • Sung Sik Kang (Hanwoo Research Institute, National Institute of Animal Science) ;
  • Hyun-Jeong Lee (Hanwoo Research Institute, National Institute of Animal Science) ;
  • Sung Jin Moon (Hanwoo Research Institute, National Institute of Animal Science) ;
  • Myung Sun Park (Hanwoo Research Institute, National Institute of Animal Science) ;
  • Hyun Tae Lim (Department of Animal Science, Gyeongsang National University) ;
  • Eun Ho Kim (Department of Animal Science, Gyeongsang National University) ;
  • Ho Chan Kang (Department of Animal Science, Gyeongsang National University) ;
  • Sun Sik Jang (Hanwoo Research Institute, National Institute of Animal Science) ;
  • Nam Young Kim (Hanwoo Research Institute, National Institute of Animal Science)
  • Received : 2023.04.14
  • Accepted : 2023.06.20
  • Published : 2023.09.01
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Abstract

The genetic diversity of three Hanwoo populations was analyzed using 11 microsatellite (MS) markers for the traceability of Hanwoo beef in this study. A total of 1,099 Hanwoo cattle from two populations (694 line-breeding and 405 general Hanwoo) at the Hanwoo Research Institute (HRI) of the National Institute of Animal Science and 1,171 Korean proven bulls (KPNs) were used for the analysis. Specific alleles of four markers (ETH10, INRA23, TGLA122, and TGLA227) were identified only in the line-breeding population, although at a low allele frequency (0.001 - 0.02). The genetic distance (Nei's D) between line-breeding Hanwoo and KPN was the greatest (0.064), whereas general Hanwoo and KPN were relatively close genetically (0.02); the distance between line-breeding and general Hanwoo was found to be 0.054. These results are expected because the HRI has performed closed breeding via selecting its line-breeding sires without utilizing KPN since 2009. Therefore, the line-breeding Hanwoo population of HRI show different genetic diversity from the KPN population, based on the 11 MS markers. The results of this study provide basic data for securing the genetic diversity of Hanwoo cattle and utilizing line-breeding Hanwoo cattle from the HRI.

Keywords

Acknowledgement

본 연구는 농촌진흥청 연구사업(과제명: 한우 미래수요대비 개량축군 조성, 유전특성 구명 및 활용기술 개발, 과제번호 : PJ01502802)에 의해 수행되었습니다.

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