Journal of Microbiology and Biotechnology

  • 제33권8호
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  • Pages.1076-1083
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  • 2023
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Poly(3-hydroxybutyrate) Degradation by Bacillus infantis sp. Isolated from Soil and Identification of phaZ and bdhA Expressing PHB Depolymerase

  • Yubin Jeon (Department of Biological and Chemical Engineering, Hongik University) ;
  • HyeJi Jin (Department of Biological and Chemical Engineering, Hongik University) ;
  • Youjung Kong (Department of Biological and Chemical Engineering, Hongik University) ;
  • Haeng-Geun Cha (Department of Biological and Chemical Engineering, Hongik University) ;
  • Byung Wook Lee (Department of Biological and Chemical Engineering, Hongik University) ;
  • Kyungjae Yu (Department of Biological and Chemical Engineering, Hongik University) ;
  • Byongson Yi (Department of Biological and Chemical Engineering, Hongik University) ;
  • Hee Taek Kim (Department of Food Science and Technology, Chungnam National University) ;
  • Jeong Chan Joo (Department of Biotechnology, The Catholic University of Korea) ;
  • Yung-Hun Yang (Department of Biological Engineering, Konkuk University) ;
  • Jongbok Lee (Department of Biological and Chemical Engineering, Hongik University) ;
  • Sang-Kyu Jung (Department of Biological and Chemical Engineering, Hongik University) ;
  • See-Hyoung Park (Department of Biological and Chemical Engineering, Hongik University) ;
  • Kyungmoon Park (Department of Biological and Chemical Engineering, Hongik University)
  • 투고 : 2023.03.12
  • 심사 : 2023.05.22
  • 발행 : 2023.08.28
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초록

Poly(3-hydroxybutyrate) (PHB) is a biodegradable and biocompatible bioplastic. Effective PHB degradation in nutrient-poor environments is required for industrial and practical applications of PHB. To screen for PHB-degrading strains, PHB double-layer plates were prepared and three new Bacillus infantis species with PHB-degrading ability were isolated from the soil. In addition, phaZ and bdhA of all isolated B. infantis were confirmed using a Bacillus sp. universal primer set and established polymerase chain reaction conditions. To evaluate the effective PHB degradation ability under nutrient-deficient conditions, PHB film degradation was performed in mineral medium, resulting in a PHB degradation rate of 98.71% for B. infantis PD3, which was confirmed in 5 d. Physical changes in the degraded PHB films were analyzed. The decrease in molecular weight due to biodegradation was confirmed using gel permeation chromatography and surface erosion of the PHB film was observed using scanning electron microscopy. To the best of our knowledge, this is the first study on B. infantis showing its excellent PHB degradation ability and is expected to contribute to PHB commercialization and industrial composting.

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과제정보

This study was supported by the National Research Foundation of Korea (NRF) (NRF-2020R1F1A1068103) and R&D Program of MOTIE/KEIT (20014350, 20015041, 20018337, and 20018132).

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