Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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The Modulatory Effect of Sodium Propionate Treatment in the Expression of Inflammatory Cytokines and Intracellular Growth of Brucella abortus 544 in Raw 264.7 Cells

  • Heejin Kim (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Tran Xuan Ngoc Huy (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Trang Thi Nguyen (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Alisha Wehdnesday Bernardo Reyes (Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Banos) ;
  • WonGi Min (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Hu Jang Lee (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Jin Hur (College of Veterinary Medicine, Chonbuk National University) ;
  • Suk Kim (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
  • Received : 2023.03.28
  • Accepted : 2023.05.12
  • Published : 2023.08.28
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Abstract

In this study, we investigated the effects of sodium propionate (SP) treatment on intracellular mechanism of murine macrophages and its contribution to host immunity during Brucella abortus 544 infection. The intracellular growth assay revealed that SP inhibited Brucella replication inside the macrophages. To determine intracellular signaling involved during SP treatment after Brucella infection, we analyzed the change of five different cytokines production relevant to SP such as TNF-α, IL-10, IFN-γ, IL-1β, and IL-6, and the results indicated that the boost with IL-10 was apparent throughout the culture period for 48 h as well as IL-1β which was apparent at 24 h post-infection and IFN-γ which was apparent at 24 h and 48 h in comparison to SP untreated groups. On the other way, SP-treated cells displayed suppressed production of TNF-α and IL-6 at all time points tested and 48 h post-infection, respectively. Furthermore, we conducted western blot to establish a cellular mechanism, and the result suggested that SP treatment attenuated p50 phosphorylation, part of the NF-κB pathway. These findings indicated that the inhibitory effect of SP against Brucella infection could be attributed through induction of cytokine production and interference on intracellular pathway, suggesting SP as a potential candidate for treating brucellosis.

Keywords

Acknowledgement

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Animal Disease Management Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (122001021SB01061382116530000).

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