Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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MiR-188-5p regulates the proliferation and differentiation of goat skeletal muscle satellite cells by targeting calcium/calmodulin dependent protein kinase II beta

  • Jing Jing (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Sihuan Zhang (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Jinbo Wei (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Yuhang Yang (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Qi Zheng (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Cuiyun Zhu (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Shuang Li (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Hongguo Cao (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Fugui Fang (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Yong Liu (Key Laboratory of Embryo Development and Reproductive Regulation of Anhui Province, Fuyang Normal University) ;
  • Ying-hui Ling (College of Animal Science and Technology, Anhui Agricultural University)
  • Received : 2023.03.13
  • Accepted : 2023.06.14
  • Published : 2023.12.01
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Abstract

Objective: The aim of this study was to reveal the role and regulatory mechanism of miR-188-5p in the proliferation and differentiation of goat muscle satellite cells. Methods: Goat skeletal muscle satellite cells isolated in the pre-laboratory were used as the test material. First, the expression of miR-188-5p in goat muscle tissues at different developmental stages was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). In addition, miR-188-5p was transfected into goat skeletal muscle satellite cells by constructing mimics and inhibitors of miR-188-5p, respectively. The changes of differentiation marker gene expression were detected by qPCR method. Results: It was highly expressed in adult goat latissimus dorsi and leg muscles, goat fetal skeletal muscle, and at the differentiation stage of muscle satellite cells. Overexpression and interference of miR-188-5p showed that miR-188-5p inhibited the proliferation and promoted the differentiation of goat muscle satellite cells. Target gene prediction and dual luciferase assays showed that miR-188-5p could target the 3'untranslated region of the calcium/calmodulin dependent protein kinase II beta (CAMK2B) gene and inhibit luciferase activity. Further functional studies revealed that CAMK2B promoted the proliferation and inhibited the differentiation of goat muscle satellite cells, whereas si-CAMK2B restored the function of miR-188-5p inhibitor. Conclusion: These results suggest that miR-188-5p inhibits the proliferation and promotes the differentiation of goat muscle satellite cells by targeting CAMK2B. This study will provide a theoretical reference for future studies on the molecular mechanisms of skeletal muscle development in goats.

Keywords

Acknowledgement

This research was supported by the National Natural Science Foundation of China (32172695), the Natural Science Foundation of Anhui Province (2108085Y11), and the 2021 annual mutton goat industry development pilot technology project of Linquan County (LQRJK2021-03) and Open project of Anhui Key Laboratory of embryonic development and reproductive regulation (FSKFKT019D).

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