DOI QR코드

DOI QR Code

Antibacterial activity of Dioscorea batatas, Morus alba, and Tagetes erecta against E. coli and S. aureus

  • Hyun-Seo Yoon (Department of Dental Hygiene, Dong-Eui University) ;
  • Chung Mu Park (Department of Clinical Laboratory Science, Dong-Eui University)
  • 투고 : 2023.08.21
  • 심사 : 2023.09.06
  • 발행 : 2023.09.30

초록

In this study, the anti-bacterial activity of three medicinal herbs such as Dioscorea batatas, Morus alba, and Tagetes erecta, have been applied in oriental medicine against Escherichia coli and Staphylococcus aureus. The antibacterial activity of Dioscorea batatas ethanol extract (DBEE), Morus alba ethanol extract (MAEE), and Tagetes erecta ethanol extract (TEEE) was analyzed by disk diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. As a result, three medicinal herbs exhibited antibacterial activity in a dose-dependent manner as well as MAEE and TEEE showed the most potent antibacterial activity followed by disk diffusion assay against E. coli and S. aureus, respectively. MIC against E. coli and S. aureus was 1.6 mg/mL of MAEE and TEEE as well as 0.2 mg/mL of TEEE. MBC against both strains was 5 mg/mL of DBEE and 2.5 mg/mL of TEEE. Consequently, three medicinal herbs in this study showed potent antibacterial activity through the inhibited growth of E. coli and S. aureus, which is considered as a potential candidate for cosmeceutic agents to attenuate the pruritus. In addition, antibacterial and antihistamine effects followed by the extraction solvents should be analyzed in future studies.

키워드

과제정보

This study was supported by Busan Technopark Foundation (2023 Busan Local Government-Industry-Academia Collaboration Corporate R&D Support Project).

참고문헌

  1. Ahn SH, Kim KB. ECS modulating effect of scutellaria baicalensis extract on inflammation relief in atopic dermatitis-induced mice. J Pediatr Korean Med. 2021. 35: 118-127.
  2. Batiha GE, Al-Snafi AE, Thuwaini MM, et al. Morus alba: a comprehensive phytochemical and pharmacological review. Naunyn Schmiedebergs Arch Pharmacol. 2023. 396: 1399-1413. https://doi.org/10.1007/s00210-023-02434-4
  3. Chan EW, Lye PY, Wong SK. Phytochemistry, pharmacology, and clinical trials of Morus alba. Chin J Nat Med. 2016. 14: 17-30.
  4. Choi EJ, Jang SR, Kang OJ, Bang WS. Antimicrobial activity of psoralea corylifolia, schisandra chinensis, and spatholobus suberectus extracts. Korean J Food Sci Technol. 2013. 45: 495-500. https://doi.org/10.9721/KJFST.2013.45.4.495
  5. Choi MH, Seo YJ, Shin HJ. Application of domestic bamboo stems mainly for inner beauty product development: A review. KSBB J. 2017. 32: 1-8. https://doi.org/10.7841/ksbbj.2017.32.1.1
  6. Hwang JW, Park CH, An HY, et al. Antibacterial, antioxidant activities of acer tegmentosum maxim ethanol extract against Staphylococcus aureus. J Plant Biotechnol. 2022. 49: 339-346. https://doi.org/10.5010/JPB.2022.49.4.339
  7. Jang DY, Yang JC. A study on the evaluation of antimicrobial activity of extracts from rhus javanica L fruit. J Kor Appl Sci Technol. 2020. 37: 145-152.
  8. Kang YM, Moon BC, Lee AY, Kim HK. Morphological characteristics and cultivation status of yams (Genus Dioscorea) as known dioscoreae rhizoma (San-yak) korean herb. Med Inf. 2014. 2: 25-31.
  9. Karadas F, Grammenidis E, Surai PF, Acamovic T, Sparks NH. Effects of carotenoids from lucerne, marigold and tomato on egg yolk pigmentation and carotenoid composition. Br Poult Sci. 2006. 47: 561-566. https://doi.org/10.1080/00071660600962976
  10. Kasiram K, Sakharkar PR, Patil AT. Antifungal activity of calendula officinalis. Indian J Pharm Sci. 2000. 62: 464-466.
  11. Kim BK, Kang JH, Oh GH, et al. Antibacterial and antioxidant activity of chamaecyparis obtusa extracts. J Life Sci. 2019. 29: 785-791.
  12. Kim DY, Cho SC, Kwon HS, Kim MK. Cosmeceutical activities of broccoli extracts. J Korea Soc Beauty Art. 2016. 17: 23-39. https://doi.org/10.18693/jksba.2016.17.4.23
  13. Kim JH, Choi YS, Kim WB, Park JO, Im DJ. Antimicrobial synergistic effects of gallnut extract and natural product mixture against human skin pathogens. J Soc Cosmet Sci Korea. 2021. 47: 155-161.
  14. Kim MH, Song CE, Park YJ. Study on the quality of marigold (Tagetes erecta L.) flower tea by its manufacture methods for expansion of utilization of marigold as tourism resources. J Kor Soc Rural Tour. 2021. 24: 75-89.
  15. Kim MK. Study on functional natural materials to improve for human health - comparison analysis of antioxidant and antimicrobial activity of fermented rutaceas fruits extract. J Soc Sci. 2021. 12: 63-78. https://doi.org/10.48033/jss.6.3.5
  16. Knor T. The pathogenesis of acne. Acta Dermatovenerol Croat. 2005. 13: 44-49.
  17. Lee JY, Park TH, Park SH, Yang SA, Jhee KH. The antimicrobial activity of fermented extracts from korean dendropanax morbifera. J Life Sci. 2019. 29: 29-36.
  18. Matsui K, Nishikawa A, Suto H, Tsuboi R, Ogawa H. Comparative study of Staphylococcus aureus isolated from lesional and nonlesional skin of atopic dermatitis patients. Microbiol Immunol. 2000. 44: 945-947. https://doi.org/10.1111/j.1348-0421.2000.tb02587.x
  19. Milligan KL, Matsui E, Sharmam H. Asthma in urban children: epidemiology, environmental risk factors, and the public health domain. Curr Allergy Asthma Rep. 2016. 16: 1-10. https://doi.org/10.1007/s11882-015-0579-0
  20. National Health Insurance Service. Status of atopic dermatitis treatment in 2021. 2022.
  21. Ogawa T, Katsuoka K, Kawano K, Nishiyama S. Comparative study of staphylococcal flora on the skin surface of atopic dermatitis patients and healthy subjects. J Dermatol. 1994. 21: 453-460. https://doi.org/10.1111/j.1346-8138.1994.tb01774.x
  22. Oh JH, Park HJ, Choi MR, Park HJ. Paraneoplastic dermatomyositis with severe itching. Kor J Dermatol. 2019. 57: 395-398.
  23. Park IH, Miin G, Kim HR, Kim JH, Hong SH. A case of liver cirrhosis patient's senile pruritus treated by external preparation containing Ponciri fructus and Radix lithospermi. J Korean Med Ophthalmol Otolaryngol Dermatol. 2016. 29: 231-239. https://doi.org/10.6114/jkood.2016.29.3.231
  24. Park MK, Cho SH, Ahn RK, et al. Immunomodulatory effects of β-sitosterol and daucosterol isolated from Dioscorea batatas on LPS-stimulated RAW 264.7 and TK-1 cells. J Life Sci. 2020. 30: 359-369.
  25. Park SH, Park HJ. Root Bark extract of Morus alba L. suppressed the migration and invasion of HCT116 human colorectal carcinoma Cells. J Physiol & Pathol Korean Med. 2021. 35: 177-184. https://doi.org/10.15188/kjopp.2021.10.35.5.177
  26. Piddock LJ. Techniques used for the determination of antimicrobial resistance and sensitivity in bacteria. J Appl Bacteriol. 1990. 68: 307-318. https://doi.org/10.1111/j.1365-2672.1990.tb02880.x
  27. Sung KC. Characteristics and analysis of natural Pine- Needles extract. J of Korean Oil Chemists Soc. 2004. 21: 320-326.
  28. Xie LR, Jun DY, Park JE, et al. Application of the extract of zanthoxylum piperitum DC to manufacturing eco-friendly antimicrobial interior fabric blind materials. J Life Sci. 2016. 26: 936-942. https://doi.org/10.5352/JLS.2016.26.8.936
  29. Yoon HS, Park CM. Inhibitory effect of persicaria perfoliata (L.) H. gross on IgE mediated allergic responses in RBL-2H3 cells. J Korean Soc Integr Med. 2020. 8: 163-169.
  30. Zitterl-Eglseer K, Sosa S, Jurenitsch J, et al. Anti-oedematous activities of the main triterpendiol esters of marigold (Calendula officinalis L.). J Ethnopharmacol. 1997. 57: 139-144. https://doi.org/10.1016/S0378-8741(97)00061-5