Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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The Quality Control Method in the Laboratory Analysis of Aquatic Ecosystem Health Monitoring and Assessment: Permanent Mounting Slides Tool Development Using Benthic Attached Diatoms.

수생태계 건강성 조사·평가를 위한 실내분석 정도관리 방법: 부착돌말류 영구표본 분석도구 개발

  • Jae-Ki Shin (Limnoecological Science Research Institute Korea (THE HANGANG)) ;
  • Nan-Young Kim (Department of Environmental Health and Science, Konkuk University) ;
  • Yongeun Park (School of Civil and Environmental Engineering, Konkuk University) ;
  • Kyung-Lak Lee (Water Environmental Research Department, National Institute of Environmental Research) ;
  • Baik-Ho Kim (Department of Life Science, Hanyang University) ;
  • Yong-Jae Kim (Department of Life Science, Daejin University) ;
  • Han-Soon Kim (Department of Biology, Kyungpook National University) ;
  • Jung Ho Lee (Department of Biology Education, Daegu University) ;
  • Hak Young Lee (Department of Biology, Chonnam National University) ;
  • Soon-Jin Hwang (Department of Environmental Health and Science, Konkuk University)
  • Received : 2023.08.07
  • Accepted : 2023.09.01
  • Published : 2023.09.30
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Abstract

Benthic attached diatoms (BADs), a major primary producer in lotic stream and river ecosystems are micro-sized organisms and require a highly magnified microscopic technique in the observation work. Thus, it is often not easy to ensure accuracy and precision in both qualitative and quantitative analyses. This study proposed a new technique applicable to improve quality control of aquatic ecosystem monitoring and assessment using BADs. In order to meet the purpose of quality control, we developed a permanent mounting slide technique which can be used for both qualitative and quantitative analyses simultaneously. We designed specimens with the combination of grid on both cover and slide glasses and compared their efficiency. As a result of observation and counting of BADs, the slide glass designed with the color-lined grid showed the highest efficiency compared to other test conditions. We expect that the method developed in this study could be effectively used to analyze BADs and contributed to improve the quality control in aquatic ecosystem health monitoring and assessment.

하천생태계의 주요 1차 생산자인 부착돌말류는 대부분 세포 크기가 매우 작아 관찰과 피각 계수에 고배율의 현미경적 방법을 요구하며, 그로 인해 정성적, 정량적 분석에 있어 정확도와 정밀도를 확보하기가 쉽지 않다. 본 연구는 부착돌말류를 이용한 수생태계 조사·평가에 있어 정도관리를 향상시키기 위한 방법을 제안하였다. 정도관리의 목적에 부합하기 위해, 정성 및 정량분석이 동시에 가능한 영구표본 슬라이드의 분석도구를 신규로 개발하였다. 영구표본을 제작함에 있어 커버 글라스와 슬라이드 글라스에 격자 유무의 조합으로 표본을 만들어 상호 특성을 비교하였다. 그 결과, 다른 시험 조건에 비해 유색선형 격자 슬라이드를 사용하는 방법이 가장 효과적이었다. 향후, 더 개선된 방법의 개발이 기대되지만, 본 연구에서 개발된 방법은 그 효율성과 적용성에 있어 기존 부착돌말류의 실내분석 정도관리를 개선하는 데 기여할 수 있을 것으로 판단된다.

Keywords

Acknowledgement

본 논문의 심사과정에서 세밀하게 검토해 주신 익명의 심사위원들께 감사드립니다.

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