대한수의학회지 (Korean Journal of Veterinary Research)

  • 제63권3호
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  • Pages.23.1-23.9
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  • 2023
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  • 2466-1384(pISSN)
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  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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육계의 조직 중 티아물린의 잔류 분석 및 휴약기간 설정 연구

Establishment of withdrawal time and analysis of tiamulin residue in tissues of orally dosed broiler chickens

  • 김우현 (경상국립대학교 수의과대학) ;
  • 김준영 (강원대학교 수의과대학) ;
  • 박광일 (경상국립대학교 수의과대학) ;
  • 진영배 (경상국립대학교 수의과대학) ;
  • 김석 (경상국립대학교 수의과대학) ;
  • 이후장 (경상국립대학교 수의과대학)
  • Woo H. Kim (College of Veterinary Medicine, Gyeongsang National University) ;
  • Jun Young Kim (College of Veterinary Medicine, Kangwon National University) ;
  • Kwang Il Park (College of Veterinary Medicine, Gyeongsang National University) ;
  • Yeung Bae Jin (College of Veterinary Medicine, Gyeongsang National University) ;
  • Suk Kim (College of Veterinary Medicine, Gyeongsang National University) ;
  • Hu-Jang Lee (College of Veterinary Medicine, Gyeongsang National University)
  • 투고 : 2023.05.27
  • 심사 : 2023.08.16
  • 발행 : 2023.09.30
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초록

This study was conducted to investigate tiamulin (TML) residues in the edible tissues of orally dosed broiler chickens and to re-establish the withdrawal time (WT). Thirty-six healthy Ross broiler chickens were administered 0.5 (TML-1) and 2.5 kg (TML-2) per ton feed, respectively, of the drug containing TML 78 g/kg for 10 days. Twenty-four tissue samples were collected from 6 chickens in each of the TML-1 and TML-2 groups on 0, 1, 3, and 5 days after drug administration, respectively. The residual concentrations of TML were measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The correlation coefficient of the calibration curves was 0.9978 to 0.9998, and the limits of detection and the limits of quantification (LOQ) were in the range of 0.03 to 0.06, and 0.1 to 0.2 ㎍/kg, respectively. Recoveries ranged between 89.0% to 116.7%, and the coefficients of variation were less than 13.9%. After the drug administration, TML in the TML-1 and TML-2 groups was detected above the LOQ in 1 and 6 samples of liver, respectively, at day 0, and in 1 liver sample from both groups on day one. At 3 days after administration, TML was detected below the LOQ in all samples of TML-1 and TML-2. The calculated WT of TML in both TML-1 and TML-2 using the WT calculation program WT 1.4 was 0 days. In conclusion, the developed analytical method is suitable for detection, and the calculated WT of TML in poultry edible tissues is shorter than the current recommended WT of 7 days for TML in broiler chickens.

키워드

과제정보

This study was supported by Livestock Health Control Association.

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