Journal of Veterinary Science

  • 제24권5호
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  • Pages.70.1-70.14
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  • 2023
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Comparison of blood parameters according to fecal detection of Mycobacterium avium subspecies paratuberculosis in subclinically infected Holstein cattle

  • Seungmin Ha (National Institute of Animal Science, Rural Development Administration) ;
  • Seogjin Kang (National Institute of Animal Science, Rural Development Administration) ;
  • Mooyoung Jung (National Institute of Animal Science, Rural Development Administration) ;
  • Sang Bum Kim (National Institute of Animal Science, Rural Development Administration) ;
  • Han Gyu Lee (National Institute of Animal Science, Rural Development Administration) ;
  • Hong-Tae Park (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University) ;
  • Jun Ho Lee (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University) ;
  • Ki Choon Choi (National Institute of Animal Science, Rural Development Administration) ;
  • Jinho Park (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk National University) ;
  • Ui-Hyung Kim (National Institute of Animal Science, Rural Development Administration) ;
  • Han Sang Yoo (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University)
  • 투고 : 2023.04.19
  • 심사 : 2023.07.27
  • 발행 : 2023.09.30
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초록

Background: Mycobacterium avium subspecies paratuberculosis (MAP) causes a chronic and progressive granulomatous enteritis and economic losses in dairy cattle in subclinical stages. Subclinical infection in cattle can be detected using serum MAP antibody enzyme-linked immunosorbent assay (ELISA) and fecal polymerase chain reaction (PCR) tests. Objectives: To investigate the differences in blood parameters, according to the detection of MAP using serum antibody ELISA and fecal PCR tests. Methods: We divided 33 subclinically infected adult cattle into three groups: seronegative and fecal-positive (SNFP, n = 5), seropositive and fecal-negative (SPFN, n = 10), and seropositive and fecal-positive (SPFP, n = 18). Hematological and serum biochemical analyses were performed. Results: Although the cows were clinically healthy without any manifestations, the SNFP and SPFP groups had higher platelet counts, mean platelet volumes, plateletcrit, lactate dehydrogenase levels, lactate levels, and calcium levels but lower mean corpuscular volume concentration than the SPFN group (p < 0.017). The red blood cell count, hematocrit, monocyte count, glucose level, and calprotectin level were different according to the detection method (p < 0.05). The SNFP and SPFP groups had higher red blood cell counts, hematocrit and calprotectin levels, but lower monocyte counts and glucose levels than the SPFN group, although there were no significant differences (p > 0.017). Conclusions: The cows with fecal-positive MAP status had different blood parameters from those with fecal-negative MAP status, although they were subclinically infected. These findings provide new insights into understanding the mechanism of MAP infection in subclinically infected cattle.

키워드

과제정보

The authors would like to express their deepest gratitude to Sanghun Park, Youngso Hong, Jihyeon Lee, Soyoung Lee, and Nuri Baek for their technical support.

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