Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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High density culture of Bacillus subtilisBSM320 in aqueous extract of composted spent mushroom substrate of Lentinula edodes and biological control of green mold disease

표고수확후배지 퇴비 물 추출물에서 Bacillus subtilis BSM 320의 고밀도 배양 및 표고 푸른곰팡이병의 생물학적 방제

  • Ja-Yoon Kim (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University) ;
  • Se-Hyun Park (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University) ;
  • Seong-Joon Park (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University) ;
  • Ju-Hyeong Hwang Bo (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University) ;
  • Hee-Wan Kang (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University)
  • 김자윤 (한경국립대학교 생명공학부 ) ;
  • 박세현 (한경국립대학교 생명공학부 ) ;
  • 박성준 (한경국립대학교 생명공학부 ) ;
  • 황보주형 (한경국립대학교 생명공학부 ) ;
  • 강희완 (한경국립대학교 생명공학부 )
  • Received : 2023.08.03
  • Accepted : 2023.09.14
  • Published : 2023.09.30
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Abstract

The objective of this study was to achieve biological control of green mold disease in Pyogo mushrooms using antagonistic microorganisms. Bacillus subtilis BSM320 cells inhibited mycelial growth by 48-60% against three Trichodermaisolates including T. hazianumisolated from the substrates of Lentinula edodes, showing their antifungal activity.The bacteria were cultured to a high density of 4.2 × 109±113.7 cfu/mlin aqueous extract of composted spent mushroom substrates of L. edodes containing 1% glucose and showed a higher growth rate than that observed when using the commercial medium, Luria-Bertani broth. The bacterial culture showed a 75% protective effect without damaging the mushroom fruiting bodies. These results suggest that B. subtilis BSM320culture is suitable for biological control of green mold disease during mushroom cultivation.

본 연구는 길항성 미생물, Bacillus subtilis BSM320을 이용한 표고 푸른곰팡이병의 생물학적 방제를 목적으로 하였다. B. subtilis BSM320는 표고 톱밥배지 에서 분리된 T. hazianum을 포함한 3 Trichoderma 분리균주 대해 균사 생장을 억제하는 항균 활성을 나타냈다. B. subtilis BSM320은 1% glucose를 포함하는 LeCSMS물 추출물(LeCSMS WE)에서 4.2 × 109±113.7 cfu/mL 세균수로 고밀도 배양 되었으며 상용배지 Lurina-Bertani broth 배지보다 높은 세균 밀도를 보였다. B. subtilis BSM320의 LeCSMS WE 배양액은 표고 자실체에 약해 없이 표고 톱밥 배지에 발생하는 푸른곰팡이병에 대하여 75%의 방제효과를 보였다. 그 결과는 LeCSMS 물 추출물 배양 B. subtilis BSM320는 표고 푸른곰팡이병의 생물학적 방제에 활용할 수 있는 것을 시사한다.

Keywords

Acknowledgement

본 연구는 농림수산식품기술기획평가원(IPET)의 유용농생명 자원산업화 기술개발 사업(과제번호:321103-3)와 중소기업벤처부 (과제번호:S3067029)의 연구지원에 의해 수행된 결과입니다.

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